Application
Immunocytochemistry
1. Fixation: 95% ethanol for 10 min
2. Washing: 1x PBS 3 min
3. Blocking: 1% BSA in PBST for 30 min
4. Primary antibody incubation (anti-vimentin AB28028): in 1% BSA in PBST for overnight (4°C)
5. Secondary antibody incubation (goat anti-mouse ab): in 1% BSA in PBST for 30 min
6. Mounting: Fluoroshield™
2. Washing: 1x PBS 3 min
3. Blocking: 1% BSA in PBST for 30 min
4. Primary antibody incubation (anti-vimentin AB28028): in 1% BSA in PBST for overnight (4°C)
5. Secondary antibody incubation (goat anti-mouse ab): in 1% BSA in PBST for 30 min
6. Mounting: Fluoroshield™
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Egi Kardia
Verified customer
提交于 Sep 11 2018