Key features and details
- Goat Anti-Mouse IgG H&L (DyLight® 488)
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, Flow Cyt
产品名称山羊抗小鼠IgG H&L (DyLight® 488)
参阅全部 IgG 二抗
特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
经测试应用适用于: IHC-P, ICC/IF, Flow Cytmore details
偶联物DyLight® 488. Ex: 493nm, Em: 518nm
存放说明Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS
Concentration information loading...
纯度Immunogen affinity purified
纯化说明This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (DyLight® 488) (ab96871) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab96871 被引用在 17 文献中.
- Jin X et al. Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways. J Orthop Surg Res 17:53 (2022). PubMed: 35093114
- Shin W & Kim HJ 3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert. Nat Protoc 17:910-939 (2022). PubMed: 35110737
- Leinweber J et al. Elastase inhibitor agaphelin protects from acute ischemic stroke in mice by reducing thrombosis, blood-brain barrier damage, and inflammation. Brain Behav Immun 93:288-298 (2021). PubMed: 33401017
- Flanagan SP et al. Nonspecific nuclear uptake of anti-MUC1 aptamers by dead cells: the role of cell viability monitoring in aptamer targeting of membrane-bound protein cancer biomarkers. Anal Methods 13:1191-1203 (2021). PubMed: 33605950
- Huang YH et al. Development of a cardiac-and-piezoelectric hybrid system for application in drug screening. Lab Chip 20:3423-3434 (2020). PubMed: 32785399