山羊抗小鼠IgG H&L (Cy5.5 ®)预吸附二抗(ab6947)

Reviews (1)Q&A (5)References (6)

Key features and details

  • Goat Anti-Mouse IgG H&L (Cy5.5 ®) preadsorbed
  • Conjugation: Cy5.5 ®. Ex: 678nm, Em: 703nm
  • Host species: Goat
  • Suitable for: ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopy

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    山羊抗小鼠IgG H&L (Cy5.5 ®)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 经测试应用

    适用于: ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr, Immunomicroscopymore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Full length native Mouse IgG (purified).

  • 偶联物

    Cy5.5 ®. Ex: 678nm, Em: 703nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

  • 共轭说明

    Cy5.5 (Cyanine 5.5-OSu) (Molecular Weight 1312 daltons) Absorption Wavelength: 678 nm Emission Wavelength: 703 nm Fluorochrome/Protein Ratio: 3.7 moles Cy5.5 per mole of Goat IgG

  • 克隆

    多克隆

  • 常规说明

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6947于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
说明
ICC/IF
1/1000 - 1/5000.
ELISA
1/10000 - 1/50000.
Flow Cyt
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (6)

发表研究结果有使用 ab6947？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6947 被引用在 6 文献中.

  • Mendez-Bermudez A  et al. Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2. Mol Cell 70:449-461.e5 (2018). PubMed: 29727617
  • Holloway PM  et al. Sulforaphane induces neurovascular protection against a systemic inflammatory challenge via both Nrf2-dependent and independent pathways. Vascul Pharmacol 85:29-38 (2016). PubMed: 27401964
  • Wilhelm T  et al. Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells. Proc Natl Acad Sci U S A 111:763-8 (2014). PubMed: 24347643
  • Miotto B  et al. The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability. Cell Rep 7:575-87 (2014). ICC/IF . PubMed: 24726359
  • Técher H  et al. Replication dynamics: biases and robustness of DNA fiber analysis. J Mol Biol 425:4845-55 (2013). PubMed: 23557832
  • Letessier A  et al. Cell-type-specific replication initiation programs set fragility of the FRA3B fragile site. Nature 470:120-3 (2011). IF . PubMed: 21258320

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Poor detection of mouse IgG in rat brain with significant background

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Image is a subsection of rat brain with ventricle and white matter tracts visible. In sections with primary antibody, a 1:125 dilution of secondary antibody resulted in very weak signal. In this image, only secondary antibody was added at the same dilution (i.e. secondary-only control) and developed a strong background signal of white matter tracts. Cell nuclei were counterstained with DAPI (blue).

Abcam user community

Verified customer

提交于 Mar 24 2021

Question

Yes, it is correct. ************does the purchasing in this group usually. I am a visiting professor here.

Feel free to contact him to double-check, he is the one who gave me the customer ID.

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Answer

Thank you for your reply.

I have sent the quote for the products requested to the email address we have on file for **********.

When you are ready to convert the quote to an order, please contact our customer service team on 1-888-772-2226 or email to mailto:us.orders@abcam.com.

If there is anything else I can help you with, please let me know.

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Question

Concerning the quote, the customer number is**********

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Answer

Thank you for your reply.

Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just wanted to make sure that the quote went to the correct person.

Thank you for your patience, and I look forward to your reply.

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Question

Can you please read my email again?

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Answer

Yes, you can use ab6947 with ab54427. The antibody that recognizes IgG will recognize all IgG subtypes, whereas the IgG1, will only recognize IgG. Therefore, if you are not intending to use any other mouse primary antibodies at the same time then the IgG antibody will work well, if however you would like to use other mouse antibodies, then I would recommend the IgG1.

Please let me know if there is anything else I can help you with, and if you can provide me the address, I can generate a quote.

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Question

I selected the following antibodies from the ABcam catalog:

Primary:

Anti-CD63 antibody [AD1] (FITC)

ab108949

$337.00



Anti-CD62P antibody [Psel.KO.2.7]

ab54427

$337.00



Anti-CD42d antibody [1C2]

ab95780

$337.00



Secondary:


Goat anti-Mouse IgG H&L (Cy5.5 ®)

ab6947

$185.00



Goat anti-Mouse IgG1 heavy chain (FITC)

ab97239

$165.00



Goat polyclonal to Armenian Hamster (TR)

ab5743

$165.00



Isotype controls:


MOUSE IgG1 antibody [MOPC-21] (FITC)

ab106163

$325.00



Mouse IgG1 [ICIGG1] - Isotype Control

ab91353

$315.00



Armenian Hamster IgG monoclonal [HTK888] - Isotype control

ab18479

$325.00



Two questions:

For the secondary antibody, will the first one (ab6947) work against ab54427, or do we need to buy the second one (ab97239)? One is against IgG, the other one is against IgG1. ab54427 is IgG1.

If you could double-check the selection for compatibility (e.g., correct isotype controls) and then ask the sales department to offer us a quote, that would be great.

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Answer

Thank you for your reply.

The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please send me your current email address and I will generate the quote.

I look forward to your reply.

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Question

Would you please tell me who is the manufacturer of this product?

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Answer

Thank you for your enquiry. We produce some of our antibodies in-house and others are sourced from suppliers whose products we believe will be of interest to our customers. This product falls into the latter category. The identity of our suppliers remains commercially sensitive information. If you have any additional questions, please contact us again.

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