Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
1% BSA + 5% NGS + 0.1% Sapo as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
None
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
Yes - saponine
Fixative
Formaldehyde
Other product details
Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 5% NGS + 0.1% Sapo
Dilution
1/50
Secondary antibody
Name
Abcam antibody:Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed
Dilution
1/300
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 24 2014