Key features and details
- Goat Anti-Mouse IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, IHC-Fr
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.1% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.788% Tris HCl, 0.0095% Magnesium chloride, 0.0014% Zinc chloride, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads.
共轭说明Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Our Abpromise guarantee covers the use of ab6790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/1000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|WB||1/1000 - 1/4000.|
|Dot blot||Use at an assay dependent concentration.|
|IHC-P||1/200 - 1/1000.|
|IHC-Fr||Use at an assay dependent concentration.|
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790) at 1/1000 dilution + Mouse IgG
Observed band size: 28,55 kDa why is the actual band size different from the predicted?
Incubated with ab6790 for 60 min at room temperature.
Blocked for 30 min at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6790 被引用在 6 文献中.
- Khalid H et al. Discovery of novel Hepatitis C virus inhibitor targeting multiple allosteric sites of NS5B polymerase. Infect Genet Evol 84:104371 (2020). PubMed: 32485331
- Popova S et al. Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal. Sci Rep 10:15185 (2020). PubMed: 32938992
- Zhao X et al. MicroRNA-939-5p directly targets IGF-1R to inhibit the aggressive phenotypes of osteosarcoma through deactivating the PI3K/Akt pathway. Int J Mol Med 44:1833-1843 (2019). PubMed: 31545400
- Li X et al. Effects of in Utero Exposure to Dicyclohexyl Phthalate on Rat Fetal Leydig Cells. Int J Environ Res Public Health 13:N/A (2016). IHC . PubMed: 26907321
- Anttila K et al. Variation in temperature tolerance among families of Atlantic salmon (Salmo salar) is associated with hypoxia tolerance, ventricle size and myoglobin level. J Exp Biol 216:1183-90 (2013). PubMed: 23487268
- Huang HY et al. The embryonic expression patterns and the knockdown phenotypes of zebrafish ADP-ribosylation factor-like 6 interacting protein gene. Dev Dyn 238:232-40 (2009). PubMed: 19097054