Application
Immunocytochemistry/ Immunofluorescence
Inner ear sensory epithelia were dissected from e16.5 mice and grown in culture media for 7 days. Then, tissues were fixed in PFA, permeabilized in trition, blocked in goat serum and incubated overnight in primary mouse anti myosin 7a antibody in 4 c. On the next morning, tissues were washed 3 times in PBS x1 and incubated for 2 hours (room temp) in Abcam's secondary goat anti mouse 594 antibody.
Tissues were imaged with Leica SP8 confocal microscope.
Staining is seen specifically in hair cells (embryonic tissues grown in media shows disorganized architecture)
Tissues were imaged with Leica SP8 confocal microscope.
Staining is seen specifically in hair cells (embryonic tissues grown in media shows disorganized architecture)
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Mr. Shahar Taiber
Verified customer
提交于 Oct 31 2018