山羊抗小鼠IgG H&L (Alexa Fluor® 405) (ab175660)

概述

  • 产品名称

    山羊抗小鼠IgG H&L (Alexa Fluor® 405)
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Mouse
  • 特异性

    This antibody is specific to Mouse IgG
  • 经测试应用

    适用于: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 偶联物

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 常规说明

    We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab175660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/200 - 1/1000.

We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) 
Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm 
Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm

Flow Cyt 1/2000.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

图片

  • ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The secondary antibody (blue) was ab175660 Alexa Fluor® 405 goat anti-mouse IgG (H+L) used at 1µg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT.

    Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG.

    This data was developed using the unconjugated antibody (ab182017).

  • Cross-reactivity of Goat anti-Mouse IgG H&L (ab182017) and Goat anti-Mouse IgG H&L obtained from two different vendors was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. This data is from a representative dilution.

    This data was developed using the unconjugated antibody (ab182017).

文献

This product has been referenced in:

  • Bernkopf DB  et al. Pgam5 released from damaged mitochondria induces mitochondrial biogenesis via Wnt signaling. J Cell Biol 217:1383-1394 (2018). Read more (PubMed: 29438981) »
  • Wu X  et al. Folic acid reverses uric acid crystal-induced surface OAT1 internalization by inhibiting RhoA activity in uric acid nephropathy. Mol Med Rep 13:2385-92 (2016). Read more (PubMed: 26846716) »
See all 2 Publications for this product

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (PFA fixed)
Confocal image of Purkinje cell (red) in cerebellum with NCX1 (green) and granule cells (blue). The cerebellar slice cultures of mouse cerebellum were fixed with 4% paraformaldehyde(6 hours at 4°C) and permeabilized with 0.5% Triton X-100. Cerebellar slices were incubated with primary antibodies (Calbindin-1:500, NCX1-1:100, and NeuN-1: 500 in PB + 3% NGS + 0.5% Triton X-100) at 4°C overnight. The secondary antibody ab175660 Alexa Fluor® 405 goat anti-mouse IgG (H+L), 1:500 was used to stain the granule cells (blue) together with other secondary antibodies Alexa 568 goat anti guinea pig for Purkinje cell (red) and Alexa 488 goat anti rabbit for NCX1 (green) at ambient temperature for 2 hours.

Dr. Pradeep Sherkhane

Verified customer

提交于 May 02 2017

Answer


ab175660 is extensively tested complete Abcam antibody i.e. it is developed in Abcam laboratory.

Please note that the Alexa Fluor conjugates are patented by life technologies so Abcam use the same conjugate under sale license.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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