山羊抗小鼠IgG H&L (ab6708)
Key features and details
- Goat Anti-Mouse IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WB
概述
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产品名称
山羊抗小鼠IgG H&L
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details -
免疫原
Mouse IgG, whole molecule
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
应用
Our Abpromise guarantee covers the use of ab6708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/150000. | |
| IP | Use at an assay dependent concentration. | |
| Immunodiffusion | Use at an assay dependent concentration. | |
| Conjugation | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | 1/3000 - 1/15000. |
图片
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg
Secondary
Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution
Observed band size: 28,55 kDa why is the actual band size different from the predicted?ab6708 incubation overnight at 4°C.
Both heavy (55kDa) and light (28kDa) chains are detected.
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Western blot - Goat Anti-Mouse IgG H&L (ab6708)All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
Lane 1 : Mouse IgG (whole molecule)
Lane 2 : Mouse IgG Fc fragment
Lane 3 : Mouse IgG Fab fragment
Lane 4 : Mouse IgM kappa
Lane 5 : Mouse serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilutionab6708 incubated for 1 hour at room temperature.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (24)
ab6708 被引用在 24 文献中.
- Feng Y et al. BATF acts as an oncogene in non-small cell lung cancer. Oncol Lett 19:205-210 (2020). PubMed: 31897131
- Li Y et al. Erastin/sorafenib induces cisplatin-resistant non-small cell lung cancer cell ferroptosis through inhibition of the Nrf2/xCT pathway. Oncol Lett 19:323-333 (2020). PubMed: 31897145
- Kuntze A et al. Protonation of Piezo1 Impairs Cell-Matrix Interactions of Pancreatic Stellate Cells. Front Physiol 11:89 (2020). PubMed: 32116794
- Li G et al. Epididymal protein 3A is upregulated and promotes cell proliferation in non-small cell lung cancer. Oncol Lett 19:4024-4030 (2020). PubMed: 32391105
- Tvrdonova M et al. Gold nanoparticles as labels for immunochemical analysis using laser ablation inductively coupled plasma mass spectrometry. Anal Bioanal Chem 411:559-564 (2019). PubMed: 30109381
