概述

  • 产品名称
    山羊抗人IgG H&L (HRP)
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to人IgG - H&L (HRP)
  • 宿主
    Goat
  • 靶标种属
    Human
  • 经测试应用
    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 免疫原

    human IgG whole molecule

  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度
    Affinity purified
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明
    Horseradish Peroxidase (HRP)
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/5000 - 1/25000.

文献

This product has been referenced in:
  • You X  et al. Evaluation of Rv0220, Rv2958c, Rv2994 and Rv3347c of Mycobacterium tuberculosis for serodiagnosis of tuberculosis. Microb Biotechnol 10:604-611 (2017). Read more (PubMed: 28217905) »
  • Atkinson KV  et al. Influenza C in Lancaster, UK, in the winter of 2014-2015. Sci Rep 7:46578 (2017). Read more (PubMed: 28406194) »

See all 6 Publications for this product

发表研究结果有使用 ab6858？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交问题

4 Q&A

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Hi, I would like to know whether this Ab cross reacts with IgGs from any other species. If yes, are there any Abs from your company that recognizes specifically human IgGs? Thank you

Anti-Human IgG antibody ab6858 has not been tested for reactivity with other species. For an antibody that is exclusively reactive with human IgG, we recommend ab97175.

Hello,   I purchased the Goat polyclonal Secondary Antibody to Human IgG - H&L (HRP) (ab6858). May I ask about the dilution factor for this antibody in ELISA? Thank you very much!   Best regards,

Thank you for contacting us. For this secondary antibody we recommend a 1/180K dilution with ELISA. However, you will need to optimise and determine the best dilutions for your specific assay; considering starting dilutions from 1/40000. I hope this in...

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I want to purchase goat anti-human IgG secondary antibody conjugated with HRP for ELISA. I want to test the autoantibodies in human serum. What is the difference between pre-adsorbed and none pre-adsorbed antibody? Which one is better for my test? Th...

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Thank you for contacting us. Pre-adsorbed antibodies are more specific towards primary antibodies than the non pre-adsorbed products. Usually, a conjugated antibody is purified by affinity chromatography using an antigen of interest coupled to ...

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Is there any difference between Human IgG antibodies ab6858 and ab7153?

Thank you for your recent telephone enquiry. I can confirm that these two antibodies are essentially the same. The difference is that ab7153 is further purified by preadsorbtion to minimize its cross reactivity to 10 serum other serum proteins. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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