山羊抗豚鼠IgG H&L (Alexa Fluor® 555) (ab150186)
Key features and details
- Goat Anti-Guinea pig IgG H&L (Alexa Fluor® 555)
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA
Related conjugates and formulations
概述
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产品名称
山羊抗豚鼠IgG H&L (Alexa Fluor® 555)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Guinea pig -
经测试应用
适用于: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISAmore details -
免疫原
The details of the immunogen for this antibody are not available.
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偶联物
Alexa Fluor® 555. Ex: 555nm, Em: 565nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
克隆
多克隆 -
同种型
IgG -
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150186于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
1/200 - 1/1000.
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Flow Cyt |
1/2000.
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/2000. |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
图片
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ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. The cells were then incubated with ab7075, rabbit anti-mouse HRP conjugated antibody, for 1h at a 1/250 dilution. The cells were then incubated with ab34580, guinea pig anti-HRP antibody, for 1h at a 1/250 dilution. Lastly, the secondary antibody (yellow) was ab150186, Goat polyclonal Secondary Antibody to Guinea pig IgG - H&L (Alexa Fluor® 555), used at 1µg/ml for 1h, as a quaternary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150186, Goat polyclonal Secondary Antibody to Guinea pig IgG - H&L (Alexa Fluor® 555), used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab150186 被引用在 11 文献中.
- Curtis GR et al. Sex-related differences in endogenous pituitary adenylate cyclase-activating polypeptide (PACAP) in the thalamic paraventricular nucleus: Implications for addiction neuroscience. Addict Neurosci 5:N/A (2023). PubMed: 36798694
- Cheret C et al. Vesicular Glutamate Transporters (SLCA17 A6, 7, 8) Control Synaptic Phosphate Levels. Cell Rep 34:108623 (2021). PubMed: 33440152
- Puentes-Mestril C et al. Sleep Loss Drives Brain Region-Specific and Cell Type-Specific Alterations in Ribosome-Associated Transcripts Involved in Synaptic Plasticity and Cellular Timekeeping. J Neurosci 41:5386-5398 (2021). PubMed: 34001629
- Horisawa-Takada Y et al. Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis. Nat Commun 12:3184 (2021). PubMed: 34075040
- Weyrer C et al. Introduction of synaptotagmin 7 promotes facilitation at the climbing fiber to Purkinje cell synapse. Cell Rep 36:109719 (2021). PubMed: 34551307