Key features and details
- Goat F(ab')2 Anti-Rat IgG - (Fab)'2 (TR), pre-adsorbed
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, Immunomicroscopy, Flow Cyt
产品名称山羊F(ab')2抗大鼠IgG - (Fab)'2 (TR),预吸附二抗
参阅全部 IgG 二抗
特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
经测试应用适用于: ICC/IF, ELISA, Immunomicroscopy, Flow Cytmore details
Cow, Horse, HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rat IgG F(ab’)2 fragment
偶联物Texas Red ®. Ex: 596nm, Em: 620nm
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituents: 1.46% Sodium chloride, 1% BSA, 0.164% Sodium phosphate
BSA is IgG and protease free
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
共轭说明Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.5 moles Texas Red per mole of Goat IgG F(ab’)2
Our Abpromise guarantee covers the use of ab6267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6267 被引用在 9 文献中.
- Xiao H et al. Metformin ameliorates bleomycin-induced pulmonary fibrosis in mice by suppressing IGF-1. Am J Transl Res 12:940-949 (2020). PubMed: 32269725
- Zheng H et al. MicroRNA-138 Inhibits Osteogenic Differentiation and Mineralization of Human Dedifferentiated Chondrocytes by Regulating RhoC and the Actin Cytoskeleton. JBMR Plus 3:e10071 (2019). PubMed: 30828688
- Zheng H et al. MicroRNA-181a/b-1 over-expression enhances osteogenesis by modulating PTEN/PI3K/AKT signaling and mitochondrial metabolism. Bone 123:92-102 (2019). PubMed: 30898695
- Li B et al. Ouabain ameliorates bleomycin induced pulmonary fibrosis by inhibiting proliferation and promoting apoptosis of lung fibroblasts. Am J Transl Res 10:2967-2974 (2018). PubMed: 30323883
- Xing W et al. Amantadine attenuates sepsis-induced cognitive dysfunction possibly not through inhibiting toll-like receptor 2. J Mol Med (Berl) 96:391-402 (2018). PubMed: 29502203
- Zhu C et al. Phytophthora capsici homologue of the cell cycle regulator SDA1 is required for sporangial morphology, mycelial growth and plant infection. Mol Plant Pathol 17:369-87 (2016). PubMed: 26095317
- Zhou X et al. Antiaging Gene Klotho Regulates Adrenal CYP11B2 Expression and Aldosterone Synthesis. J Am Soc Nephrol 27:1765-76 (2016). PubMed: 26471128
- Louis ED et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett 518:49-54 (2012). PubMed: 22561033
- Ørom UA et al. MicroRNA-10a binds the 5'UTR of ribosomal protein mRNAs and enhances their translation. Mol Cell 30:460-71 (2008). PubMed: 18498749