山羊F(ab')2抗兔IgG - H&L (HRP),预吸附二抗(ab6013)
Key features and details
- Goat F(ab')2 Anti-Rabbit IgG - H&L (HRP), pre-adsorbed
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHC
Related conjugates and formulations
概述
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产品名称
山羊F(ab')2抗兔IgG - H&L (HRP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins. -
经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details -
最小交叉反应
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
免疫原
Rabbit IgG, whole molecule
-
偶联物
HRP
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/500 - 1/2500.
|
|
Dot blot |
Use at an assay dependent dilution.
|
|
WB | (1) |
1/2000 - 1/10000.
|
ELISA |
1/115000.
|
|
Electron Microscopy |
Use at an assay dependent dilution.
|
|
IHC |
Use at an assay dependent dilution.
|
说明 |
---|
ICC/IF
1/500 - 1/2500. |
Dot blot
Use at an assay dependent dilution. |
WB
1/2000 - 1/10000. |
ELISA
1/115000. |
Electron Microscopy
Use at an assay dependent dilution. |
IHC
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (7)
ab6013 被引用在 7 文献中.
- Chinn AM et al. PDE4B Is a Homeostatic Regulator of Cyclic AMP in Dendritic Cells. Front Pharmacol 13:833832 (2022). PubMed: 35387344
- Verona M et al. Preliminary Study of a 1,5-Benzodiazepine-Derivative Labelled with Indium-111 for CCK-2 Receptor Targeting. Molecules 26:N/A (2021). PubMed: 33572353
- Tan L et al. Arousing Effects of Electroacupuncture on the "Shuigou Point" in Rats with Disorder of Consciousness after Traumatic Brain Injury. Evid Based Complement Alternat Med 2021:6611461 (2021). PubMed: 33959185
- Ibrahim B et al. Albumin promotes proliferation of G1 arrested serum starved hepatocellular carcinoma cells. PeerJ 8:e8568 (2020). PubMed: 32185103
- Westcott NP et al. Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress. Nat Chem Biol 13:302-308 (2017). PubMed: 28092360
- Chou MH et al. Effects of hepatocyte CD14 upregulation during cholestasis on endotoxin sensitivity. PLoS One 7:e34903 (2012). WB . PubMed: 22511970
- Fuhs SR & Insel PA Caveolin-3 undergoes SUMOylation by the SUMO E3 ligase PIASy: sumoylation affects G-protein-coupled receptor desensitization. J Biol Chem 286:14830-41 (2011). PubMed: 21362625