Key features and details
- Goat F(ab')2 Anti-Rabbit IgG - H&L (Biotin), pre-adsorbed
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopy
产品名称山羊F(ab')2抗兔IgG - H&L (Biotin),预吸附二抗
参阅全部 IgG 二抗
特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
经测试应用适用于: ICC/IF, IHC-P, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, SheepTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Rabbit IgG whole molecule
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
共轭说明Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Our Abpromise guarantee covers the use of ab6012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|IHC-P||Use at an assay dependent dilution. PubMed: 21498392|
|Dot blot||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
|ELISA||1/20000 - 1/100000.|
|IHC||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6012 被引用在 5 文献中.
- Webb TE et al. An internal ribosome entry site in the 5' untranslated region of epidermal growth factor receptor allows hypoxic expression. Oncogenesis 4:e134 (2015). WB ; Human . PubMed: 25622307
- Huang R et al. Association between human cytomegalovirus infection and histone acetylation level in various histological types of glioma. Oncol Lett 10:2812-2820 (2015). IHC . PubMed: 26722247
- Wang Z et al. Changes in large pulmonary arterial viscoelasticity in chronic pulmonary hypertension. PLoS One 8:e78569 (2013). IHC (PFA fixed) . PubMed: 24223157
- Kacevska M et al. Extrahepatic cancer suppresses nuclear receptor-regulated drug metabolism. Clin Cancer Res 17:3170-80 (2011). IHC-P . PubMed: 21498392
- Chamberlain G et al. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS ONE 3:e2934 (2008). Flow Cyt . PubMed: 18698345