Key features and details
- Goat F(ab')2 Anti-Rabbit IgG Fc (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy, IP
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
共轭说明Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab’)2 molecule
Our Abpromise guarantee covers the use of ab6021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|Dot blot||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
|ELISA||1/20000 - 1/100000.|
|IHC||Use at an assay dependent dilution.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6021 被引用在 1 文献中.
- Lee JH et al. 53BP1 promotes ATM activity through direct interactions with the MRN complex. EMBO J : (2009). IP . PubMed: 20010693