山羊F(ab')2抗兔IgG F(ab')2 (HRP)预吸附二抗(ab6112)

Submit a review Q&A (2)References (18)

Key features and details

  • Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHC

概述

  • 产品名称

    山羊F(ab')2抗兔IgG F(ab')2 (HRP)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Rabbit

  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins..

  • 经测试应用

    适用于: ICC/IF, IHC-P, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details

  • 最小交叉反应


    Cow, Horse, Human, Mouse, Rat, Sheep more details

  • 免疫原

    Rabbit IgG F(ab’)2 fragment

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 共轭说明

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
IHC-P 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA 1/100000.

This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/2000 of the reconstitution concentration is suggested for this product. Suitable for other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Electron Microscopy Use at an assay dependent concentration.
IHC Use at an assay dependent dilution.

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)

    IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab6112, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
    Western blot - Goat F(ab')2 Anti-Rabbit IgG F(ab')2 (HRP) preadsorbed (ab6112)
    Ab6112 was used at dilution 1/5000 with the primary antibody ab32117 in WB. See the review on ab32117.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (18)

发表研究结果有使用 ab6112？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6112 被引用在 18 文献中.

  • Dubrovina AS  et al. Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA. Int J Mol Sci 20:N/A (2019). PubMed: 30934883
  • Deng Z  et al. lncRNA ATXN8OS promotes breast cancer by sequestering miR-204. Mol Med Rep 20:1057-1064 (2019). PubMed: 31173245
  • Liu X  et al. Expression of cleaved caspase-3 predicts good chemotherapy response but poor survival for patients with advanced primary triple-negative breast cancer. Int J Clin Exp Pathol 11:4363-4373 (2018). PubMed: 31949833
  • Gao L  et al. Tumor associated macrophages induce epithelial to mesenchymal transition via the EGFR/ERK1/2 pathway in head and neck squamous cell carcinoma. Oncol Rep 40:2558-2572 (2018). PubMed: 30132555
  • Yang R  et al. LASP2 suppressed malignancy and Wnt/ß-catenin signaling pathway activation in bladder cancer. Exp Ther Med 16:5215-5223 (2018). PubMed: 30542477
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Question

How about if this antibody ab8888 (anti-Dopamine) conduct for mouse brain sample IHC experiment?
Besides, customer consider use monoclonal antibody such as ab8892(anti-Dopamine) use for mouse sample IHC.
Customer concern cross reaction of secondary anibody.
Which secondary antibody you suggest since their brain sample also from mouse.
Thanks a lot for your assistance.
Best regards,

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Answer

Thank you for contacting us.

If you are using a Rabbit primary antibody in Mouse sections, the recommended secondary must be an anti-Rabbit raised preferably in a species which is as phylogenetically different as possible (distant) from mouse species to avoid cross reactivity.

We have some secondaries in our catalogue raised in Goat that could be appropriate for your assay:

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-ab-2-F-ab-2-Fragment-ab6112.html

https://www.abcam.com/Goat-F-ab-2-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Biotin-pre-adsorbed-ab6012.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-F-c-F-ab-2-Fragment-ab6021.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-F-ab-2-Fragment-ab6013.html

https://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-Fab-Fragment-ab7171.html

It is also very important to use a blocking agent such as serum from the same species as the secondary was raised in. If you used any of the secondaries suggested, therefore, 10% goat serum would be the most suitable blocking agent.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information

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Question

Ab6112 - is the buffer supposed to contain sodium azide, or is this a mistake? Also, why are these two antibodies being sold at the same price as the recommended dilutions for ELISA are different?

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Answer

Thank you for your enquiry. I apologize as there was a mistake on the datasheet for ab6112 - the antibody storage buffer contains 0.01% Gentamicin sulfate and not sodium azide. I have updated the datasheet to reflect this. The immunogen used to generate ab6112 was Rabbit IgG F(ab')2 fragment. The immunogen used to generate ab6013 was Rabbit IgG, whole molecule. This is the difference between these two antibodies. All of our secondary antibodies are priced at $150.00. It is up to you to decide which antibody better suits your needs. If you have any additional questions, please contact us again.

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