山羊F(ab')2抗小鼠IgM mu chain (Texas Red ®) (ab5927)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (Texas Red ®)
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA
概述
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产品名称
山羊F(ab')2抗小鼠IgM mu chain (Texas Red ®)
参阅全部 IgM 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. -
经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details -
免疫原
Mouse IgM mu chain
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偶联物
Texas Red ®. Ex: 596nm, Em: 620nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.4 moles Texas Red per mole of Goat IgG F(ab’)2 -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000 - 1/5000.
|
|
| Immunomicroscopy |
Use at an assay dependent dilution.
|
|
| Flow Cyt |
Use at an assay dependent dilution.
|
|
| ELISA |
1/10000 - 1/50000.
|
| 说明 |
|---|
|
ICC/IF
1/1000 - 1/5000. |
|
Immunomicroscopy
Use at an assay dependent dilution. |
|
Flow Cyt
Use at an assay dependent dilution. |
|
ELISA
1/10000 - 1/50000. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (4)
ab5927 被引用在 4 文献中.
- Kaur P et al. Expression profiling of RNA transcripts during neuronal maturation and ischemic injury. PLoS One 9:e103525 (2014). ICC/IF . PubMed: 25061880
- Radan L et al. Microenvironmental regulation of telomerase isoforms in human embryonic stem cells. Stem Cells Dev 23:2046-66 (2014). Flow Cyt . PubMed: 24749509
- Encinas G et al. Detection of four germ cell markers in rats during testis morphogenesis: differences and similarities with mice. Cells Tissues Organs 195:443-55 (2012). PubMed: 21893932
- Lineburg KE et al. The carbohydrate CT1 is expressed in topographically fixed glomeruli in the mouse olfactory bulb. Mol Cell Neurosci 48:9-19 (2011). PubMed: 21699983
