山羊F(ab')2抗小鼠IgM mu chain (PE) (ab5932)
Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (PE)
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt
概述
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产品名称
山羊F(ab')2抗小鼠IgM mu chain (PE)
参阅全部 IgM 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectro-phoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgM, and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or human, bovine or horse serum proteins. -
经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cytmore details -
免疫原
Mouse IgM mu heavy chain
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偶联物
PE. Ex: 488nm, Em: 575nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
R-Phycoerythrin (R-PE) (Molecular Weight 240,000 daltons) from seaweed Absorption Wavelength: 490 nm, 545 nm and 565 nm Emission Wavelength: 580 nm OD566nm/OD280nm: 2.6 Phycobiliprotein Concentration: 0.5 mg/ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound) -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/250.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications. |
|
Flow Cyt |
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
|
说明 |
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ICC/IF
1/100 - 1/250. |
Immunomicroscopy
Use at an assay dependent dilution. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications. |
Flow Cyt
1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab5932 被引用在 3 文献中.
- Sahu G et al. Junctophilin Proteins Tether a Cav1-RyR2-KCa3.1 Tripartite Complex to Regulate Neuronal Excitability. Cell Rep 28:2427-2442.e6 (2019). PubMed: 31461656
- Chen J & Liu F Analysis of ABO chimera from peripheral red cells and reticulocytes by flow cytometry and micro gel column technique in patients post-ABO-incompatible HSCT. Clin Transplant 32:N/A (2018). PubMed: 29226393
- Lappalainen J et al. A protocol for generating high numbers of mature and functional human mast cells from peripheral blood. Clin Exp Allergy 37:1404-14 (2007). Flow Cyt ; Mouse . PubMed: 17845422