Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHC
产品名称山羊F(ab')2抗小鼠IgM mu chain (HRP)
参阅全部 IgM 二抗
特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
经测试应用适用于: ICC/IF, IHC-Fr, Dot blot, WB, ELISA, Electron Microscopy, IHCmore details
Mouse IgM mu chain
存放说明Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.87% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
共轭说明Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Our Abpromise guarantee covers the use of ab5930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/5000.|
|IHC-Fr||Use at an assay dependent dilution.|
|Dot blot||1/5000 - 1/100000.|
|WB||1/2000 - 1/10000.|
|Electron Microscopy||Use at an assay dependent dilution.|
|IHC||1/500 - 1/5000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5930 被引用在 9 文献中.
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- Hasham MG et al. Systemic autoimmunity induced by the TLR7/8 agonist Resiquimod causes myocarditis and dilated cardiomyopathy in a new mouse model of autoimmune heart disease. Dis Model Mech 10:259-270 (2017). ELISA . PubMed: 28250051
- Rieder LE et al. Dynamic response of RNA editing to temperature in Drosophila. BMC Biol 13:1 (2015). PubMed: 25555396
- Kuge H et al. Functional compartmentalization of the plasma membrane of neurons by a unique acyl chain composition of phospholipids. J Biol Chem 289:26783-93 (2014). ELISA . PubMed: 25096572
- Oksenberg N et al. Function and regulation of AUTS2, a gene implicated in autism and human evolution. PLoS Genet 9:e1003221 (2013). PubMed: 23349641
- Orihuela CJ et al. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. J Clin Invest : (2009). IHC-Fr ; Mouse . PubMed: 19436113
- Shanley DK & Sullivan AM Expression of the cell surface markers mAb 2F7 and PSA-NCAM in the embryonic rat brain. Neurosci Lett 424:165-9 (2007). WB ; Mouse . PubMed: 17723277