Key features and details
- Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cyt
产品名称山羊F(ab')2抗小鼠IgM mu chain (Biotin)
参阅全部 IgM 二抗
特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
经测试应用适用于: ICC/IF, ELISA, WB, Dot blot, IHC, Immunomicroscopy, Flow Cytmore details
Mouse IgM mu heavy chain
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.3% Potassium phosphate, 1% BSA
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
共轭说明Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG F(ab')2 molecule
Our Abpromise guarantee covers the use of ab5929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/2500.|
|ELISA||1/10000 - 1/50000.|
|WB||1/1000 - 1/5000.|
|Dot blot||Use at an assay dependent dilution.|
|IHC||1/500 - 1/5000.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|Flow Cyt||1/200 - 1/1000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5929 被引用在 1 文献中.
- Grewal N et al. Accumulation of oxidized LDL in the tendon tissues of C57BL/6 or apolipoprotein E knock-out mice that consume a high fat diet: potential impact on tendon health. PLoS One 9:e114214 (2014). IHC . PubMed: 25502628