Key features and details
- Goat F(ab')2 Anti-Mouse IgG+IgM+IgA H&L (TRITC) preadsorbed
- Conjugation: TRITC. Ex: 547nm, Em: 572nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA
产品名称山羊F(ab')2抗小鼠IgG+IgM+IgA H&L (TRITC)预吸附二抗
参阅全部 IgG + IgM + IgA 二抗
特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Human Serum Proteins. This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
经测试应用适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details
HumanTo ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Mouse IgG, IgA, and IgM whole molecules
偶联物TRITC. Ex: 547nm, Em: 572nm
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free
Concentration information loading...
纯化说明This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
共轭说明Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 3.5 moles TRITC per mole of Goat IgG
Our Abpromise guarantee covers the use of ab6004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/1000 - 1/5000.|
|Immunomicroscopy||Use at an assay dependent dilution.|
|Flow Cyt||1/500 - 1/2500.|
|ELISA||1/10000 - 1/50000.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.