山羊F(ab')2抗小鼠IgG H&L (PE)预吸附二抗(ab7002)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG H&L (PE) preadsorbed
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr
概述
-
产品名称
山羊F(ab')2抗小鼠IgG H&L (PE)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Frmore details -
最小交叉反应
Cow, Hamster, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Full length native Mouse IgG (purified).
-
偶联物
PE. Ex: 488nm, Em: 575nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation -
共轭说明
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 -
克隆
多克隆 -
同种型
IgG -
常规说明
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
-
研究领域
应用
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/250.
|
|
Immunomicroscopy |
Use at an assay dependent dilution.
|
|
Flow Cyt |
1/100 - 1/250. The maximum amount of Ab need to stain 106 cells in flow cytometry is 1.0 ug.
|
|
IHC-P |
Use at an assay dependent dilution.
|
|
IHC-Fr |
Use at an assay dependent dilution.
|
说明 |
---|
ICC/IF
1/100 - 1/250. |
Immunomicroscopy
Use at an assay dependent dilution. |
Flow Cyt
1/100 - 1/250. The maximum amount of Ab need to stain 106 cells in flow cytometry is 1.0 ug. |
IHC-P
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab7002 被引用在 5 文献中.
- Hill PJ et al. Modifications of Pseudomonas aeruginosa cell envelope in the cystic fibrosis airway alters interactions with immune cells. Sci Rep 7:4761 (2017). Flow Cyt . PubMed: 28684799
- Ju L et al. Von Willebrand factor-A1 domain binds platelet glycoprotein Iba in multiple states with distinctive force-dependent dissociation kinetics. Thromb Res 136:606-12 (2015). PubMed: 26213126
- Bârsan L et al. Bone marrow iron distribution, hepcidin, and ferroportin expression in renal anemia. Hematology 20:543-52 (2015). PubMed: 25745821
- Cai D et al. Improved tools for the Brainbow toolbox. Nat Methods 10:540-7 (2013). PubMed: 23817127
- Schulz U et al. Interleukin-10 Promotes NK Cell Killing of Autologous Macrophages by Stimulating Expression of NKG2D Ligands. Scand J Immunol 72:319-31 (2010). Flow Cyt . PubMed: 20883317