山羊F(ab')2抗小鼠IgG - H&L (AP),预吸附二抗(ab5871)
Key features and details
- Goat F(ab')2 Anti-Mouse IgG - H&L (AP), pre-adsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC
概述
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产品名称
山羊F(ab')2抗小鼠IgG - H&L (AP),预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Mouse -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. -
经测试应用
适用于: ICC/IF, Dot blot, WB, ELISA, IHCmore details -
最小交叉反应
Cow, Horse, Human, Rabbit, Rat, Sheep more details -
免疫原
Mouse IgG whole molecule.
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偶联物
Alkaline Phosphatase
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
应用
Our Abpromise guarantee covers the use of ab5871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/200 - 1/1000. | |
Dot blot | Use at an assay dependent dilution. | |
WB | 1/500 - 1/2500. | |
ELISA | 1/9600. | |
IHC | Use at an assay dependent dilution. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
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