山羊F(ab')2抗小鼠IgG (Fab')2 (Texas Red ®)预吸附二抗(ab5884)

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Key features and details

  • Goat F(ab')2 Anti-Mouse IgG (Fab')2 (Texas Red ®) preadsorbed
  • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA

概述

  • 产品名称

    山羊F(ab')2抗小鼠IgG (Fab')2 (Texas Red ®)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details

  • 最小交叉反应


    Cow, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Mouse IgG F(ab)2 fragment

  • 偶联物

    Texas Red ®. Ex: 596nm, Em: 620nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 共轭说明

    Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.7 moles Texas Red per mole of Goat IgG F(ab’)2

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab5884？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5884 被引用在 3 文献中.

  • Mobarra N  et al. Three-dimensional nanofiberous PLLA/PCL scaffold improved biochemical and molecular markers hiPS cell-derived insulin-producing islet-like cells. Artif Cells Nanomed Biotechnol 46:S685-S692 (2018). PubMed: 30284475
  • Allahverdi A  et al. Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1. Cell J 17:231-42 (2015). PubMed: 26199902
  • Jafarian A  et al. The Generation of Insulin Producing Cells from Human Mesenchymal Stem Cells by MiR-375 and Anti-MiR-9. PLoS One 10:e0128650 (2015). IF . PubMed: 26047014

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Question

Dear Sir, 1. Please describe the problem (high background, no staining etc). No staining 2. On what material are you testing the antibody in IHC? Species? Cell line? Lymphoblast (Human) Tissue? 3. How did you fix the samples? Ethanol, methanol (Tried) Acetone (Tried) Paraformaldehyde Other (10%TCA) 4. Did you apply antigen retrieval step? Enzymatic method Heat mediated technique Other 5. How did you block the unspecific binding sites? Using 10%BSA in PBS+ 0.1% Tween 20 6. Primary antibody Specification (in which species was it raised against)? Mouse At what dilution(s) have you tested this antibody? 1:100, 1:500 and 1:1000 Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 2h. 5min washing with PBST for 15min. 7. Secondary antibody What secondary antibody are you using? anti-mouse (TR-labelled) Specification (in which species was it raised against)? goat At what dilution(s) have you tested this antibody? 1:100, 500 & 1000 Incubation, wash steps? 0.5h and 1h at R.T and 37C Do you know whether the problems you are experiencing come from the secondary? yes, since I do get signal using another aliquote of the treated cells but using another secondary Ab. What detection method are you using? Fluoresence microscopy. 8. Background staining Please provide an image of your staining I do not have a picture (As it is almost faint red with no indication about cells. However I do get very nice stainíng from DAPI. 9. Which detection system did you use? Fluoresence microscopy. 10. Did you apply positive and negative controls along with the samples? Please specify. Yes, DAPI staining to prove that I have cells on the slides. double staining the same slide with another antibody against another target and it works. 11. Optimization attempts How many times have you tried the IHC? 15 times Do you obtain the same results every time? What steps have you altered? Fixation, primary and secondary dilutions I am looking forward to hearing from you soon. Thank you for your offer. Yes I do like to try it. You can send it to the below address:

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Answer

Thank you for your e-mail and for providing more information of your protocol. I have placed an order for your -one vial of replacement vial of ab5884. For your information, the new order number is: 40004. Please do let me know how you are getting on with this replacement.

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Question

Thank you for your E-mail. I have placed an order on 5th of january 2004. I have started to use TR-antibdoy( ab5884) and after a lot of trials I did not get any specificsignal. Please below are the answers to your questions. 1. What is the primary antibody you used? Monoclonal Antibody to Poly(ADP-ribose) What is the Ig subclass of this product? IgG3 2. Did you purchase the primary from Abcam? No..I have purchased two batches from two different companies: Alexis and Trevign. I tried them alone (since both of them are mouse) or in combination and it did not work. Also, If it works I get a signal in the treated cells as well as non-treated cells. This signal is also present in the sample where I omitted the primary antibody. It is clear the the secondary antibody is not working and cross react with something else. I have tried to dilute the secondary antibody more to attenuate the non-specific binding but when I do that I totally lose the signal. 3. Is the primary antibody compatible with the secondary antibody? Yes, the primary antibody is mouse anti-PAR and the seconadary is goat anti-mouse (TR-labelled) I look forward to hearing from you soon. Thank you for your fruitful co-operation. Best regards,

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Answer

Thank you for your quick reply and your answers to our questions. We can offer you a replacement vial - free of charge. Please do let us know if you would like to try and test it. We are looking forward to hearing from you soon.

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