山羊F(ab')2抗小鼠IgG - F(ab')2 (HRP),预吸附二抗(ab5887)

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Key features and details

  • Goat F(ab')2 Anti-Mouse IgG - F(ab')2 (HRP), pre-adsorbed
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopy

概述

  • 产品名称

    山羊F(ab')2抗小鼠IgG - F(ab')2 (HRP),预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

  • 经测试应用

    适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details

  • 最小交叉反应


    Cow, Horse, Human, Rabbit, Rat, Sheep more details

  • 免疫原

    Mouse IgG F(ab)2 fragment

  • 偶联物

    HRP

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and protease free
  • Concentration information loading...

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

  • 共轭说明

    Horseradish Peroxidase (HRP) (Molecular Weight 40,000 daltons)

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent dilution.
WB 1/2000 - 1/20000.
ELISA 1/100000. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
IHC Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (3)

发表研究结果有使用 ab5887？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab5887 被引用在 3 文献中.

  • Hosokawa M  et al. Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy. iScience 13:229-242 (2019). PubMed: 30870781
  • Kii I  et al. Selective inhibition of the kinase DYRK1A by targeting its folding process. Nat Commun 7:11391 (2016). PubMed: 27102360
  • Chen ST  et al. CLEC5A regulates Japanese encephalitis virus-induced neuroinflammation and lethality. PLoS Pathog 8:e1002655 (2012). PubMed: 22536153

客户评价及客户问答

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Question

I am looking for a HRP conjugated anti-mouse antibody to be used in ELISA. The most important thing is that it will have the least cross reactivity to rat immunoglobulins. I see that you have many such antibodies. Which one would you think is the most suitable?


Thanks,

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Answer

Thank you for contacting us.

The following products would be suitable as per your requirements. The catalogue numbers are

ab5870, ab5887, ab7061, ab7068, ab98717, ab98790 etc

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Thank you for your information in the previous email. I have an enquiry about the secondary antibody-HRP. What's the difference between ab5887 and ab5870?

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Answer

Thank you for your question. Both antibodies are only a F(ab)2 Fragment of antibody itself (therefore these antibody will not give F(c) mediated binding), but they different in the immunogens used and therefore epitopes recognized: Ab5887 was raised against Mouse IgG F(ab)2 fragment. Ab5870 was raised against Mouse IgG whole molecule. I hope this information will help. Please do not hesitate to contact us if you need further assistance.

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Question

May I ask once more? My samples are sheep proteins. I am going to use two antibodies (anti GAPDH and anti GDF8 antibodies). The first antibodies are raised in mouse and rat (mouse IgG and Rat IgG), respectively. There are many secondary antibodies (HRP conjugated) which recognize mouse IgG or rat IgG (ex. ab5870, ab5879, ab5887, ab6728.....). As some of them are raised in goat, I am afraid that I will have a high background. I would like to know which secondary antibodies against mouse and rat IgG is good for western blotting to detect sheep proteins. Thank you for your helps.

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Answer

Thank you for your enquiry. Given that you are using mouse and rat primary antisera and would like to use a secondary antiserum that will not detect any endogenous IgG in your sheep samples, bearing in mind a potential cross reactivity with goat, I would like to recommend the following: ab6728 Mouse IgG antibody (ab6728) HRP conjugated Rabbit ab6820 Mouse IgG antibody (ab6820) HRP conjugated Donkey Both antisera have been raised in rabbit or donkey and are highly unlikely to cross react against sheep. We have also received favourable reviews for them both.

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