山羊F(ab)抗兔IgG H&L (FITC) (ab7050)
Key features and details
- Goat F(ab) Anti-Rabbit IgG H&L (FITC)
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC/IF, Immunomicroscopy, Flow Cyt, ELISA
Related conjugates and formulations
概述
-
产品名称
山羊F(ab)抗兔IgG H&L (FITC)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rabbit -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). -
经测试应用
适用于: ICC/IF, Immunomicroscopy, Flow Cyt, ELISAmore details -
免疫原
Rabbit IgG whole molecule
-
偶联物
FITC. Ex: 493nm, Em: 528nm
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. -
共轭说明
Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 4.0 moles FITC per mole of Goat IgG Fab -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| Immunomicroscopy | ||
| Flow Cyt | ||
| ELISA |
| 说明 |
|---|
应用说明
Flow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependent dilution.
Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (5)
ab7050 被引用在 5 文献中.
- Balinas C et al. Identification and characterisation of transient receptor potential melastatin 2 and CD38 channels on natural killer cells using the novel application of flow cytometry. BMC Immunol 20:14 (2019). PubMed: 31077146
- Balinas C et al. Transient receptor potential melastatin 2 channels are overexpressed in myalgic encephalomyelitis/chronic fatigue syndrome patients. J Transl Med 17:401 (2019). PubMed: 31796045
- Scherz B et al. mTh1 driven expression of hTDP-43 results in typical ALS/FTLD neuropathological symptoms. PLoS One 13:e0197674 (2018). PubMed: 29787578
- Zhang J et al. Hsp60 exerts a tumor suppressor function by inducing cell differentiation and inhibiting invasion in hepatocellular carcinoma. Oncotarget 7:68976-68989 (2016). IF . PubMed: 27677587
- Zheng JP et al. NF-kappaB signaling mediates vascular smooth muscle endothelin type B receptor expression in resistance arteries. Eur J Pharmacol 637:148-54 (2010). IHC . PubMed: 20399772
