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Secondary antibodies anti-Chicken IgY Enzyme HRP

山羊抗鸡IgY H&L (HRP) (ab6877)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (44)

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Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

Key features and details

  • Goat Anti-Chicken IgY H&L (HRP)
  • Conjugation: HRP
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

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概述

  • 产品名称

    山羊抗鸡IgY H&L (HRP)
    参阅全部 IgY 二抗
  • 宿主

    Goat
  • 靶标种属

    Chicken
  • 经测试应用

    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 免疫原

    chicken IgY whole molecule

  • 偶联物

    HRP

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate, 1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Horseradish Peroxidase (HRP)
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Enzyme
    • HRP

相关产品

  • Substrate reagent

    • TMB ELISA Substrate (Highest Sensitivity) (ab171522)
    • TMB ELISA Substrate (High Sensitivity) (ab171523)
    • TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
    • TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
    • TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
    • TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6877于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/1000 - 1/10000.
Dot blot
Use at an assay dependent dilution.
ELISA
1/50000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
WB (1)
1/2000 - 1/20000. PubMed: 16543490
说明
ICC/IF
1/1000 - 1/10000.
Dot blot
Use at an assay dependent dilution.
ELISA
1/50000.
IHC-P
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Immunomicroscopy
Use at an assay dependent dilution.
WB
1/2000 - 1/20000. PubMed: 16543490

图片

  • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)Lichtenfels et al PLoS One. 2012;7(7):e41345. doi: 10.1371/journal.pone.0041345. Epub 2012 Jul 20. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Representative Western blot analysis for the selected panel of differentially expressed targets in the various T cell subsets.

    Western blot analyses were performed by serially probing sets of 3 membranes per donor. The expression levels of GDIR1, GSTO1, LIMS1 and PROF1 were analyzed blot 1 (upper panel), GDIR2, PRDX2 and THIO on blot 2 (middle panel) and SODM on blot 3 (lower level) of the respective membrane set.

    The relative expression levels were defined by densitometric quantification normalized to the corresponding beta-actin signals, co-detected on each membrane.

    Aliquots of 15 µg total lysate per sample/lane were loaded onto Tris-Tricine gels (16%T/2.5%C) and subsequently separated. Lysates generated form untreated CD45RA+ and CD45RO+ T cells were throughout the set of membranes loaded onto the lanes 1 and 3 respectively, whereas lysates generated from the corresponding T cell subsets exposure for 3 h to 5 µM H2O2 were loaded onto the lanes 2 and 4 as indicated.

    The protein entry names of the targets (left) and their corresponding molecular weight (right) are listed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

    IHC image of beta Actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab13822, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6877, 1/500 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

    ab6877 was used at dilution 1/1000 with the primary antibody ab25912 in WB. See the review on ab25912.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

    ab6877 was used at dilution 1/200 with the primary antibody ab17517 in IHC-P. See the review on ab17517.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (44)

发表研究结果有使用 ab6877?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6877 被引用在 44 文献中.

  • Albanese M  et al. Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4+ T cells allows complex functional analyses. Nat Methods 19:81-89 (2022). PubMed: 34949807
  • Lu L  et al. Selective cleavage of ncRNA and antiviral activity by RNase2/EDN in THP1-induced macrophages. Cell Mol Life Sci 79:209 (2022). PubMed: 35347428
  • Fu Y  et al. Downregulation of secreted protein acidic and rich in cysteine in human trabecular meshwork cells. Exp Ther Med 22:1126 (2021). PubMed: 34504578
  • Zhang K  et al. Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis. Front Mol Neurosci 14:777621 (2021). PubMed: 34867191
  • Chandrasekaran S  et al. Neuron-specific chromosomal megadomain organization is adaptive to recent retrotransposon expansions. Nat Commun 12:7243 (2021). PubMed: 34903713
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Human iPSC-derived neurons MAP2

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Protein extracts from human iPSC-derived neurons were separated on the SDS PAGE and immunoblotted with MAP2 antibody (ab5392, 1:40 000 dilution). Secondary goat anti chicken antibody (ab6877) were used at 1:10 000 dilution.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

提交于 Nov 30 2020

Question

Is AB 6877 IGG or IGY? The website says IGY so a customer ordered this product from us. The lable on the vial however says IGG but has the correct item number (6877). Was this an error on the label or the website? Thanks much

Read More

Abcam community

Verified customer

Asked on Sep 06 2002

Answer

This antibody is a goat IgG anti-chicken IgY. We apologise for any mistakes on the vial label.

Read More

Abcam Scientific Support

回复于 Sep 09 2002

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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