山羊抗鸡IgY H&L (Biotin) (ab6876)
Key features and details
- Goat Anti-Chicken IgY H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgY
- Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB
概述
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产品名称
山羊抗鸡IgY H&L (Biotin)
参阅全部 IgY 二抗 -
宿主
Goat -
靶标种属
Chicken -
经测试应用
适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details -
免疫原
chicken IgY whole molecule
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偶联物
Biotin
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
纯度
IgY fraction -
纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule -
克隆
多克隆 -
同种型
IgY -
研究领域
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/3000.
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| Dot blot |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent dilution.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
|
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| Immunomicroscopy |
Use at an assay dependent concentration.
|
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| WB |
1/3000 - 1/30000.
|
| 说明 |
|---|
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ICC/IF
1/500 - 1/3000. |
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Dot blot
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent dilution. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Immunomicroscopy
Use at an assay dependent concentration. |
|
WB
1/3000 - 1/30000. |
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)This image is courtesy of an Abreview submitted by Carl Hobbs.
ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab6876 被引用在 6 文献中.
- Forbes D et al. Liquid Dermal Scaffold With Adipose-Derived Stem Cells Improve Tissue Quality in a Murine Model of Impaired Wound Healing. J Burn Care Res 40:550-557 (2019). PubMed: 31188436
- Wang L et al. Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of a-synuclein and neuroinflammation. Cell Death Dis 9:440 (2018). PubMed: 29670081
- Huang B et al. Dysregulation of Notch and ERa signaling in AhR-/- male mice. Proc Natl Acad Sci U S A 113:11883-11888 (2016). IHC . PubMed: 27688768
- Huang B et al. Differential expression of estrogen receptor a, ß1, and ß2 in lobular and ductal breast cancer. Proc Natl Acad Sci U S A 111:1933-8 (2014). PubMed: 24449868
- Yang Z et al. {beta}-Agonist-associated Reduction in RGS5 Expression Promotes Airway Smooth Muscle Hyper-responsiveness. J Biol Chem 286:11444-55 (2011). IHC-Fr . PubMed: 21278382
- Muthusamy S et al. Estrogen receptor ß and 17ß-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer. Proc Natl Acad Sci U S A 108:20090-4 (2011). IHC-P . PubMed: 22114194