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Secondary antibodies anti-Chicken IgY Biotin

山羊抗鸡IgY H&L (Biotin) (ab6876)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (9)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
  • Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)

Key features and details

  • Goat Anti-Chicken IgY H&L (Biotin)
  • Conjugation: Biotin
  • Host species: Goat
  • Isotype: IgY
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

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概述

  • 产品名称

    山羊抗鸡IgY H&L (Biotin)
    参阅全部 IgY 二抗
  • 宿主

    Goat
  • 靶标种属

    Chicken
  • 经测试应用

    适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
  • 免疫原

    chicken IgY whole molecule

  • 偶联物

    Biotin

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度

    IgY fraction
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
  • 克隆

    多克隆
  • 同种型

    IgY
  • 研究领域

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Biotin

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6876于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/500 - 1/3000.
Dot blot
Use at an assay dependent concentration.
ELISA
Use at an assay dependent dilution.
IHC-P (1)
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/3000 - 1/30000.
说明
ICC/IF
1/500 - 1/3000.
Dot blot
Use at an assay dependent concentration.
ELISA
Use at an assay dependent dilution.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
WB
1/3000 - 1/30000.

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)This image is courtesy of an Abreview submitted by Carl Hobbs.

    ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in  TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.

  • Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
    Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.
    Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (9)

发表研究结果有使用 ab6876?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6876 被引用在 9 文献中.

  • Huang HT  et al. Hericium erinaceus mycelium and its small bioactive compounds promote oligodendrocyte maturation with an increase in myelin basic protein. Sci Rep 11:6551 (2021). PubMed: 33753806
  • Huijgens PT  et al. Silencing and stimulating the medial amygdala impairs ejaculation but not sexual incentive motivation in male rats. Behav Brain Res 405:113206 (2021). PubMed: 33639266
  • Doornaert M  et al. Xenogen-free isolation and culture of human adipose mesenchymal stem cells. Stem Cell Res 40:101532 (2019). PubMed: 31421383
  • Forbes D  et al. Liquid Dermal Scaffold With Adipose-Derived Stem Cells Improve Tissue Quality in a Murine Model of Impaired Wound Healing. J Burn Care Res 40:550-557 (2019). PubMed: 31188436
  • Wang L  et al. Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of a-synuclein and neuroinflammation. Cell Death Dis 9:440 (2018). PubMed: 29670081
  • Huang B  et al. Dysregulation of Notch and ERa signaling in AhR-/- male mice. Proc Natl Acad Sci U S A 113:11883-11888 (2016). IHC . PubMed: 27688768
  • Huang B  et al. Differential expression of estrogen receptor a, ß1, and ß2 in lobular and ductal breast cancer. Proc Natl Acad Sci U S A 111:1933-8 (2014). PubMed: 24449868
  • Yang Z  et al. {beta}-Agonist-associated Reduction in RGS5 Expression Promotes Airway Smooth Muscle Hyper-responsiveness. J Biol Chem 286:11444-55 (2011). IHC-Fr . PubMed: 21278382
  • Muthusamy S  et al. Estrogen receptor ß and 17ß-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer. Proc Natl Acad Sci U S A 108:20090-4 (2011). IHC-P . PubMed: 22114194

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

GFP expresssing Mouse astrocytes in brain

Excellent
Abreviews
Abreviews
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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed paraffin wax section was subjected to HIER before incubating in anti GFP ( ab13970). Section was then incubated with ab6876 ( 1/200) followed by stABCpx. GFP was visualised using DAB chromogen

MR. Carl Hobbs

Verified customer

提交于 Sep 25 2013

Question

I would like to know were is exacli attached the biotin. Is it only in the Fc region of the antibody?

Read More

Abcam community

Verified customer

Asked on Jun 16 2006

Answer

I have been informed that amine reactive biotins were used to conjugate Lysine sites of antibodies. It is not only in the Fc region. Please do not hesitate to contact us again if you need more information,

Read More

Abcam Scientific Support

回复于 Jun 19 2006

Question

I am thinking of using this antibody as a negative control for a biotinylated goat polyclonal Ab. Have you got any data regarding cross-reactions with human, rabbit and mouse proteins (especially Ig)?

Read More

Abcam community

Verified customer

Asked on Oct 16 2003

Answer

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. At this time, we do not have any data about cross-reactivity with human, rabbit and mouse proteins.

Read More

Abcam Scientific Support

回复于 Oct 16 2003

Question

Is this Antibody compartible (regarding sensivity) with Antirabbit IgG-Biotin from DAKO?

Read More

Abcam community

Verified customer

Asked on May 31 2001

Answer

We don't have any data on the sensitivity of antibodies from DAKO, so its' difficult to compare. We do know that this antibody recognises 1µg chicken IgG at a working dilution of at least 1/20000.

Read More

Abcam Scientific Support

回复于 May 31 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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