山羊抗鸡IgY H&L (Alexa Fluor® 750)预吸附二抗(ab175755)
Key features and details
- Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed
- Conjugation: Alexa Fluor® 750. Ex: 749nm, Em: 775nm
- Host species: Goat
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA, WB
Related conjugates and formulations
概述
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产品名称
山羊抗鸡IgY H&L (Alexa Fluor® 750)预吸附二抗
参阅全部 IgY 二抗 -
宿主
Goat -
靶标种属
Chicken -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
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经测试应用
适用于: IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA, WBmore details -
最小交叉反应
Cow, Horse, Human, Mouse, Pig, Rabbit, Rat more details -
免疫原
The details of the immunogen for this antibody are not available.
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偶联物
Alexa Fluor® 750. Ex: 749nm, Em: 775nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
常规说明
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175755于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
This applicaiton is no longer batch tested |
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ICC/IF |
1/200 - 1/1000.
This applicaiton is no longer batch tested
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Flow Cyt |
1/2000.
This applicaiton is no longer batch tested |
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IHC-P |
Use at an assay dependent concentration.
This applicaiton is no longer batch tested |
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ELISA |
Use at an assay dependent concentration.
This applicaiton is no longer batch tested |
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WB |
1/10000.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. This applicaiton is no longer batch tested |
ICC/IF
1/200 - 1/1000. This applicaiton is no longer batch tested
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Flow Cyt
1/2000. This applicaiton is no longer batch tested |
IHC-P
Use at an assay dependent concentration. This applicaiton is no longer batch tested |
ELISA
Use at an assay dependent concentration. This applicaiton is no longer batch tested |
WB
1/10000. |
图片
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Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (ab175755) at 1/10000 dilution
Observed band size: 37 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab83956 overnight at 4°C. Antibody binding was detected using ab175755 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab175755 被引用在 1 文献中.
- Hofstee MI et al. A murine Staphylococcus aureus fracture-related infection model characterised by fracture non-union, staphylococcal abscess communities and myeloid-derived suppressor cells. Eur Cell Mater 41:774-792 (2021). PubMed: 34151416