概述

  • 产品名称

    山羊抗鸡IgY H&L (Alexa Fluor® 750)
    参阅全部 IgY 二抗
  • 宿主

    Goat
  • 靶标种属

    Chicken
  • 特异性

    This antibody is specific to Chicken IgY
  • 经测试应用

    适用于: WBmore details
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 偶联物

    Alexa Fluor® 750. Ex: 749nm, Em: 775nm

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.02% Sodium azide
    Constituents: 23% Glycerol, PBS, 1% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • 克隆

    多克隆
  • 常规说明

    We batch test Goat Anti-Chicken IgY H&L (Alexa Fluor® 750), ab175754 in fluorescent WB. Although we don’t batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Chicken IgY H&L (Alexa Fluor® 750), ab175754 in these applications.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab175754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000.

图片

  • Anti-GAPDH antibody - Loading Control (ab83956) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) (ab175754) at 1/10000 dilution

    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab83956 overnight at 4°C. Antibody binding was detected using ab175754 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

文献

This product has been referenced in:

  • Ma Y  et al. Inhibiting tumor necrosis factor-a signaling attenuates postoperative cognitive dysfunction in aged rats. Mol Med Rep 12:3095-100 (2015). Read more (PubMed: 25955232) »
See 1 Publication for this product

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There are currently no Customer reviews or Questions for ab175754.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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