山羊抗亚美尼亚仓鼠IgG H&L (Biotin) (ab5744)
Key features and details
- Goat Anti-Armenian hamster IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, Dot blot, ELISA, Immunomicroscopy, IHC-P
概述
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产品名称
山羊抗亚美尼亚仓鼠IgG H&L (Biotin)
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Armenian hamster -
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum. Cross reactivity against mouse has not been determined. -
经测试应用
适用于: WB, ICC/IF, Dot blot, ELISA, Immunomicroscopy, IHC-Pmore details -
免疫原
Armenian Hamster IgG whole molecule
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偶联物
Biotin
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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纯化说明
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
共轭说明
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
应用
Our Abpromise guarantee covers the use of ab5744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/2000 - 1/10000. | |
ICC/IF | 1/1000 - 1/5000. | |
Dot blot | Use at an assay dependent dilution. | |
ELISA | 1/250000. | |
Immunomicroscopy | Use at an assay dependent dilution. | |
IHC-P | Use at an assay dependent dilution. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (8)
ab5744 被引用在 8 文献中.
- Zheng J et al. Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes. BMC Cancer 18:608 (2018). PubMed: 29848296
- Dinh CH et al. Bardoxolone Methyl Prevents High-Fat Diet-Induced Colon Inflammation in Mice. J Histochem Cytochem 64:237-55 (2016). PubMed: 26920068
- Onodera K et al. GATA2 regulates dendritic cell differentiation. Blood 128:508-18 (2016). PubMed: 27259979
- Dinh CH et al. Bardoxolone Methyl Prevents Mesenteric Fat Deposition and Inflammation in High-Fat Diet Mice. ScientificWorldJournal 2015:549352 (2015). IHC-P . PubMed: 26618193
- Dinh CH et al. Bardoxolone Methyl Prevents Fat Deposition and Inflammation in Brown Adipose Tissue and Enhances Sympathetic Activity in Mice Fed a High-Fat Diet. Nutrients 7:4705-23 (2015). IHC-P . PubMed: 26066016
- Accart N et al. Revisiting fixation and embedding techniques for optimal detection of dendritic cell subsets in tissues. J Histochem Cytochem 62:661-71 (2014). PubMed: 24874853
- Yu P et al. Diet-induced obesity drives negative mouse vein graft wall remodeling. J Vasc Surg N/A:N/A (2013). PubMed: 23876511
- Dahllöf MS et al. The lysine deacetylase inhibitor Givinostat inhibits ß-cell IL-1ß induced IL-1ß transcription and processing. Islets 4:417-22 (0). PubMed: 23486342