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Immunology Immunoglobulins Heavy Chain IgG

山羊抗亚美尼亚仓鼠IgG H&L (ab5738)

  • Datasheet
Submit a review Q&A (3)References (1)

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Key features and details

  • Goat Anti-Armenian hamster IgG H&L
  • Host species: Goat
  • Isotype: IgG
  • Suitable for: ELISA, WB, ICC/IF, IP, Immunodiffusion, Conjugation

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 568 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase Biotin FITC Texas Red ® TRITC

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概述

  • 产品名称

    山羊抗亚美尼亚仓鼠IgG H&L
    参阅全部 IgG 二抗
  • 宿主

    Goat
  • 靶标种属

    Armenian hamster
  • 经测试应用

    适用于: ELISA, WB, ICC/IF, IP, Immunodiffusion, Conjugationmore details
  • 免疫原

    Armenian Hamster IgG whole molecule.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Hamster
    • IgG
    • Unconjugated

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5738于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/20000 - 1/100000.
WB
1/2000 - 1/10000.
ICC/IF
1/1000 - 1/5000.
IP
Use at an assay dependent dilution.
Immunodiffusion
Use at an assay dependent dilution.
Conjugation
Use at an assay dependent dilution.
说明
ELISA
1/20000 - 1/100000.
WB
1/2000 - 1/10000.
ICC/IF
1/1000 - 1/5000.
IP
Use at an assay dependent dilution.
Immunodiffusion
Use at an assay dependent dilution.
Conjugation
Use at an assay dependent dilution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab5738?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5738 被引用在 1 文献中.

  • Prenek L  et al. Regulatory T cells are less sensitive to glucocorticoid hormone induced apoptosis than CD4+ T cells. Apoptosis 25:715-729 (2020). PubMed: 32737651

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Inquiry: I am looking to purchase the product 173004. I would like to know if you have any information on the cross-reactivtiy of this antibody, specifically with mouse immunoglobulins including the IgE light chain. My cell sample will be labeled with a fluorescent mouse IgE prior to labeling with the anti-CD49e and then with product 173004. Thank you for any information you may be able to provide.

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Abcam community

Verified customer

Asked on Oct 30 2014

Answer

The antibody is not tested against IgE specifically so the degree of possible cross reactivity is not known, but it is not at a level that can be detected by immunoelectrophoresis. No reactivity with other Igs appears in our testing against armenian hamster serum.

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Kevin Hanson

Abcam Scientific Support

回复于 Oct 30 2014

Question

I apologize it was so long for me to get back to you.  We have decided to go ahead with the antibodies you suggested, ab33483, ab6640 and ab8918.  I believe it will be difficult to use all three on a single tissue sample so we are going to proceed with serial sectioning immunohistochemistry instead of fluorescent staining, hopefully with the paraffin embedded sections although I saw that the mannose receptor suggested against this.  I believe biotin secondaries are the most common for these applications?  Again I am pretty new to this procedure.  So we will need to order one for hamster, rat, and mouse if you may recommend them.  Also, if you have any opinions on a better conjugation choice or procedure choice feel free to suggest.  Thanks for your help! any way.

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Abcam community

Verified customer

Asked on Jun 05 2012

Answer

Thank you for contacting us. I am very glad to hear that you will be using our products in your experiments. I can gladly recommend three biotin labeled antibodies to suit your needs and have placed links for biotin labeled antibodies below. I would like to offer a second option which will allow you to perform these experiments but will allow you future flexibility as well. I recommend purchase of unlabeled secondary antibodies and labeling these with the marker of your choice (biotin, fluorochrome) using our EasyLink direct conjugation kits. In this way you will be able to perform serial stains using a single primary/secondary combination and will be able to optimize and trust them in this assay. You will also have the ability to use these same antibodies, apply different labels and use them in different assays such as a double or triple staining with fluorescent tags, western blot with HRP or other assays. Our EasyLink antibody conjugation kits provide asimple and quick linking process whichis part of a new generation of conjugation procedures. These eliminate many of the difficulties which were common in previous generation of conjugation kits andallow a quick and easy process to conjugate an antibody of your choice, in whatever function you need. It has the advantage of being aone-stepantibody labeling method withno separation steps. Further information about our EasyLink kits may be found at the following links: Landing Page:https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink FAQs:https://www.abcam.com/index.html?pageconfig=resource&rid=13156 The unconjugated antibodies which I would recommend would be: ab98800, anti-mouse, unconjugated:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-pre-adsorbed-ab98800.html ab98383, anti-rat, unconjugated:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-pre-adsorbed-ab98383.html ab5738, anti-Armenian hamster, unconjugated:https://www.abcam.com/Armenian-Hamster-IgG-secondary-antibody-H-L-ab5738.html For your initial biotin labeling I would recommend one of the following EasyLink Biotin (type A) labeling kits: 3 x10ug size:https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-10%C2%B5g-Biotin-ab102865.html 3 x 100ug size:https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100%C2%B5g-Biotin-ab102864.html The pre-conjugated biotin labeled antibodies can be found at the following links: ab98802 anti-mouse, biotin:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Biotin-pre-adsorbed-ab98802.html ab98414 anti-rat, biotin:https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-Biotin-pre-adsorbed-ab98414.html ab5744 anti-Armenian hamster, biotin:https://www.abcam.com/Armenian-Hamster-IgG-secondary-antibody-H-L-ab5744.html Finally, as the anti-mannose receptor antibody has not yet been tested in IHC-P, I would like to offer to you enrollment in our 100% Abreview testing discount program. DISCOUNT CODE: XXXXXXX Expiration date: XXXXX There is no obligation for you to participate in this program; however should you wish to participate this code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-P with ab8918 and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Jun 05 2012

Question

I am interested in purchasing a large batch (200-400 mg) of unconjugated goat anti-hamster IgGs with specificity to both Armenian and Syrian hamsters, preferably without sodium azide, for use in a high throughput primary cell differentiation assay. 

Read More

Abcam community

Verified customer

Asked on Nov 02 2011

Answer

Thank you for contacting us. We currently have five unconjugated goat polyclonal antibodies raised against either Syrian or Armenian hamster IgG. All have azide but will likely be available without azide as a custom order. As far as we know, reactivity with both species has not been assessed. I am looking into whether this has been checked in each case and will send an update once I find out, hopefully later today. Here is a list of the antibodies: ab6887 ab102304 ab5738 There are also two that have been pre-adsorbed to remove reactivity with IgG of other species but Syrian and Armenian hamster reactivity has not been adsorbed: ab7141 ab102314  

Read More

Abcam Scientific Support

回复于 Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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