Anti-GNB1抗体(ab137635)
Key features and details
- Rabbit polyclonal to GNB1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GNB1抗体
参阅全部 GNB1 一抗 -
描述
兔多克隆抗体to GNB1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow, Dog, Xenopus laevis, Xenopus tropicalis -
免疫原
Recombinant fragment, corresponding to a region within amino acids 67-271 of Human GNB1.
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阳性对照
- A431 whole cell lysate (ab7909) can be used as a positive control in WB. Jurkat whole cells lysate; Mouse brain whole cell lysate; methanol-fixed HeLa cells; Lung papillory Adeno Carcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137635于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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WB |
1/5000 - 1/20000. Predicted molecular weight: 37 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
WB
1/5000 - 1/20000. Predicted molecular weight: 37 kDa. |
靶标
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功能
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. -
序列相似性
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats. -
翻译后修饰
Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP. - Information by UniProt
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数据库链接
- Entrez Gene: 2782 Human
- Entrez Gene: 14688 Mouse
- Entrez Gene: 24400 Rat
- Omim: 139380 Human
- SwissProt: P62871 Cow
- SwissProt: P62873 Human
- SwissProt: P62874 Mouse
- SwissProt: P54311 Rat
see all -
别名
- Beta subunit signal transducing proteins GS/GI antibody
- G protein beta 1 subunit antibody
- GBB1 antibody
see all
图片
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Anti-GNB1 antibody (ab137635) at 1/5000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 37 kDa
10% SDS Page -
Immunohistochemical analysis of paraffin-embedded human Lung papillory Adeno Carcinoma, labelling GNB1 using ab137635 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labelling GNB1 using ab137635 at 1/200 dilution. Lower panel represents cells costained with Hoechst 33342.
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Anti-GNB1 antibody (ab137635) at 1/10000 dilution + Mouse brain whole cells lysate at 50 µg
Predicted band size: 37 kDa
10% SDS Page
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab137635 被引用在 3 文献中.
- Tang W et al. AFAP1 antisense RNA 1 promotes retinoblastoma progression by sponging microRNA miR-545-3p that targets G protein subunit beta 1. Bioengineered 13:5638-5652 (2022). PubMed: 35193469
- Huang C et al. PAX8-AS1 knockdown facilitates cell growth and inactivates autophagy in osteoblasts via the miR-1252-5p/GNB1 axis in osteoporosis. Exp Mol Med 53:894-906 (2021). PubMed: 34012023
- Murakami T et al. Cutting Edge: G Protein Subunit ß 1 Negatively Regulates NLRP3 Inflammasome Activation. J Immunol 202:1942-1947 (2019). PubMed: 30777924