重组Anti-GM130抗体[EP892Y] - BSA and Azide free (ab215966)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP892Y] to GM130 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB
- Reacts with: Dog, Human, African green monkey
Related conjugates and formulations
概述
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产品名称
Anti-GM130抗体[EP892Y] - BSA and Azide free
参阅全部 GM130 一抗 -
描述
兔单克隆抗体[EP892Y] to GM130 - BSA and Azide free -
宿主
Rabbit -
特异性
Mouse and rat cell lines pc12, 3t3, raw 264.7 were tested positive in WB. However, brain, kidney, spleen and heart were negative from the two species.
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经测试应用
适用于: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Dog, Human, African green monkey
预测可用于: Cow, Monkey不与反应: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, MCF7, MDCK(NBL-2), MDBK(BL-1) and COS-1 cell lysates. IHC-P: Human cervix carcinoma and liver tissues. ICC/IF: HeLa and MCF7 cells.
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常规说明
ab215966 is the carrier-free version of ab52649.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP892Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 647 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab195303)
- Alexa Fluor® 488 Anti-GM130 antibody [EP892Y] (ab275987)
- PE Anti-GM130 - cis-Golgi Marker antibody [EP892Y] (ab303011)
- APC Anti-GM130 - cis-Golgi Marker antibody [EP892Y] (ab303012)
- HRP Anti-GM130 - cis-Golgi Marker antibody [EP892Y] (ab303013)
- Alexa Fluor® 555 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab311890)
- Alexa Fluor® 568 Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab312361)
- Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab215966于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
PFA fixation should be most suitable. |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. Overnight incubation is recommended. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 112 kDa).
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PFA fixation should be most suitable. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. Overnight incubation is recommended. |
WB
Use at an assay dependent concentration. Detects a band of approximately 140 kDa (predicted molecular weight: 112 kDa). |
靶标
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功能
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. -
序列相似性
Belongs to the GOLGA2 family. -
结构域
Extended rod-like protein with coiled-coil domains. -
细胞定位
Golgi apparatus > Golgi stack membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2801 Human
- Omim: 602580 Human
- SwissProt: Q08379 Human
- Unigene: 155827 Human
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别名
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
图片
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Effect of Irgm1 palmitoylation mutation on Golgi association
Irgm1 KO MEF were transfected with plasmids expressing wild-type or mutant Irgm1 proteins, as indicated. The cells were exposed to 100 U/ml IFN-γ for 24 h, stained with anti-Irgm1 and anti-GM130 antibodies, and used for immunofluorescence analysis. The experiment was performed 3 times, with at least 20 cells analyzed per group in each experiment. (A) Shown are images from representative cells. The scale bar represents 20 µm.
Cells are 4% paraformaldehyde fixed, 0.2% saponin-permeabilized.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GM130 (red) with ab52649 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling GM130 with purified ab52649 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GM130 with purified ab52649 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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ab52649 (purified) at 1/20 immunoprecipitating GM130 in HeLa whole cell lysate.
Lane 1 (input): HeLa whole cell lysate (10µg)
Lane 2 (+): ab52649 + HeLa whole cell lysate (10µg).
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52649 in HeLa whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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Unpurified ab52649 staining GM130 (magenta) in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. Nuclei stained with Picogreen.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GM130 with unpurified ab52649 at a dilution of 1/500.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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Unpurified ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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Unpurified ab52649 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37°C. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4°C. An undiluted Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
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ICC/IF image of unpurified ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab52946, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (27)
ab215966 被引用在 27 文献中.
- Chen X et al. Exosomal Long Non-coding RNA HOTTIP Increases Resistance of Colorectal Cancer Cells to Mitomycin via Impairing MiR-214-Mediated Degradation of KPNA3. Front Cell Dev Biol 8:582723 (2020). PubMed: 33585440
- Raza S et al. A bovine herpesvirus 1 pUL51 deletion mutant shows impaired viral growth in vitro and reduced virulence in rabbits. Oncotarget N/A:N/A (2016). PubMed: 26934330
- Mazzulli JR et al. a-Synuclein-induced lysosomal dysfunction occurs through disruptions in protein trafficking in human midbrain synucleinopathy models. Proc Natl Acad Sci U S A 113:1931-6 (2016). PubMed: 26839413
- Klünder S et al. Site-1 protease-activated formation of lysosomal targeting motifs is independent of the lipogenic transcription control. J Lipid Res 56:1625-32 (2015). Mouse . PubMed: 26108224
- Sin YY et al. Small heat shock protein 20 (Hsp20) facilitates nuclear import of protein kinase D 1 (PKD1) during cardiac hypertrophy. Cell Commun Signal 13:16 (2015). PubMed: 25889640