Glutathione Sepharose® (ab193267)
概述
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产品名称
Glutathione Sepharose® -
产品概述
Contents:
Supplied as a 50 % slurry in 20 % Ethanol / PBS; > 3 mg (10 µmol) glutathione per mL Sepharose® beads.
Features:
The Glutathione-S-Transferase (GST) gene fusion system has been widely used for the overexpression of foreign genes in E. Coli and other expression systems. The GST-tagged protein can be easily purified from cell lysate or media by affinity chromatography using glutathione Sepharose® beads. Abcam’s Glutathione Sepharose® is designed for the purification of GST fusion proteins and other glutathione binding proteins. This formulation exhibits excellent binding capacity, high flow rate, no significant loss of the glutathione ligand and a pH stability range of 2-10.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
Purification of GST-fusion proteins or other glutathione-binding proteins.
Sepharose is a registered trademark of GE Healthcare
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说明
This product is manufactured by BioVision, an Abcam company and was previously called 6555 Glutathione Sepharose. 6555-10 is the same size as the 10 ml size of ab193267.
Glutathione Sepharose® is prepared by covalently coupling glutathione to epoxy-activated 4% cross-linked Sepharose® beads to form a stable thioether linkage. The coupling was optimized to give a high binding capacity, which could be greater than 5 mg of Glutathione-S-transferase (GST) per ml of wet gel.
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经测试应用
适用于: Purificationmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 ml 10 ml 50 ml Glutathione Sepharose® 1 x 1ml 1 x 10ml 1 x 50ml -
研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193267于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Purification |
Use at an assay dependent concentration.
Purification of GST-fusion proteins or other glutathione-binding proteins. |
说明 |
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Purification
Use at an assay dependent concentration. Purification of GST-fusion proteins or other glutathione-binding proteins. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab193267 被引用在 2 文献中.
- Wang W et al. Hemidesmosomes modulate force generation via focal adhesions. J Cell Biol 219:N/A (2020). PubMed: 31914171
- Dagliyan O et al. Engineering proteins for allosteric control by light or ligands. Nat Protoc 14:1863-1883 (2019). PubMed: 31076662