重组Anti-glutathione S transferase Omega 1/p28抗体[EPR8370(B)] (ab129106)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8370(B)] to glutathione S transferase Omega 1/p28
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-glutathione S transferase Omega 1/p28抗体[EPR8370(B)]
参阅全部 glutathione S transferase Omega 1/p28 一抗 -
描述
兔单克隆抗体[EPR8370(B)] to glutathione S transferase Omega 1/p28 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human glutathione S transferase Omega 1/p28 aa 50-150. The exact sequence is proprietary.
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阳性对照
- WB: Human fetal liver, HEK293T, Jurkat, BxPC3, HepG2 and HeLa lysates. IHC-P: Human colon tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8370(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129106于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid. -
组织特异性
Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung. -
序列相似性
Belongs to the GST superfamily. Omega family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. -
细胞定位
Cytoplasm > cytosol. - Information by UniProt
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数据库链接
- Entrez Gene: 9446 Human
- Entrez Gene: 114846 Rat
- Omim: 605482 Human
- SwissProt: P78417 Human
- SwissProt: Q9Z339 Rat
- Unigene: 190028 Human
- Unigene: 25166 Rat
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别名
- AA407097 antibody
- AI194287 antibody
- AU018802 antibody
see all
图片
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All lanes : Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : GSTO1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaLanes 1-4: Merged signal (red and green). Green - ab129106 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129106 Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] was shown to specifically react with glutathione S transferase Omega 1/p28 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266277 (knockout cell lysate ab257982) was used. Wild-type and glutathione S transferase Omega 1/p28 knockout samples were subjected to SDS-PAGE. ab129106 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab129106, at 1/100 dilution, staining glutathione S transferase Omega 1/p28 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-glutathione S transferase Omega 1/p28 antibody [EPR8370(B)] (ab129106) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : BxPC3 lysate
Lane 3 : HepG2 lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab129106 被引用在 2 文献中.
- Wang K et al. Glutathione S-transferase ? 1 promotes the proliferation, migration and invasion, and inhibits the apoptosis of non-small cell lung cancer cells, via the JAK/STAT3 signaling pathway. Mol Med Rep 23:N/A (2021). PubMed: 33236161
- Zimmerman AD et al. Changes in hepatic phase I and phase II biotransformation enzyme expression and glutathione levels following atrazine exposure in female rats. Xenobiotica 48:867-881 (2018). PubMed: 28882082