Application
Western blot
Sample
Human Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
35 µg
Specification
neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1X TBS,0.1% Tween-20,5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/6000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
Additional data
Additional Notes
145kDa band in the image shows vinculin (loading control) stained separately and GPX4 in the bottom around 17kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 23 2022