Anti-Glutathione Peroxidase 2/GPX2抗体(ab64322)
Key features and details
- Rabbit polyclonal to Glutathione Peroxidase 2/GPX2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Glutathione Peroxidase 2/GPX2抗体
参阅全部 Glutathione Peroxidase 2/GPX2 一抗 -
描述
兔多克隆抗体to Glutathione Peroxidase 2/GPX2 -
宿主
Rabbit -
特异性
Reacts specifically with human 22 kDa Glutathione Peroxidase 2/GPX2 protein
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经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Glutathione Peroxidase 2/GPX2.
Database link: P18283 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64322于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/1000.
|
说明 |
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IHC-P
1/1000. |
靶标
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功能
Could play a major role in protecting mammals from the toxicity of ingested organic hydroperoxides. Tert-butyl hydroperoxide, cumene hydroperoxide and linoleic acid hydroperoxide but not phosphatidycholine hydroperoxide, can act as acceptors. -
组织特异性
Mostly in liver and gastrointestinal tract, not found in heart or kidney. -
序列相似性
Belongs to the glutathione peroxidase family. -
细胞定位
Cytoplasm. Mainly cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 2877 Human
- Entrez Gene: 14776 Mouse
- Entrez Gene: 29326 Rat
- Omim: 138319 Human
- SwissProt: P18283 Human
- SwissProt: Q9JHC0 Mouse
- SwissProt: P83645 Rat
- Unigene: 2704 Human
see all -
别名
- Gastrointestinal glutathione peroxidase antibody
- GI GPx antibody
- Glutathione peroxidase 2 (gastrointestinal) antibody
see all
图片
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Ab64322 staining human normal bladder tissue. Staining is localized to cytoplasm.
Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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Datasheet download
文献 (3)
ab64322 被引用在 3 文献中.
- Chavatte L et al. Elemental and molecular imaging of human full thickness skin after exposure to heavy metals. Metallomics 12:1555-1562 (2020). PubMed: 32851388
- Cecchini MJ et al. CDX2 and Muc2 immunohistochemistry as prognostic markers in stage II colon cancer. Hum Pathol 90:70-79 (2019). PubMed: 31121192
- Liu T et al. GPX2 overexpression indicates poor prognosis in patients with hepatocellular carcinoma. Tumour Biol 39:1010428317700410 (2017). PubMed: 28635398