重组
RabMAb

Anti-Glutathione Peroxidase 1抗体[EPR3312] (Alexa Fluor® 488) (ab197527)

概述

  • 产品名称
    Anti-Glutathione Peroxidase 1抗体[EPR3312] (Alexa Fluor® 488)
    参阅全部 Glutathione Peroxidase 1 一抗
  • 描述
    兔单克隆抗体[EPR3312] to Glutathione Peroxidase 1 (Alexa Fluor® 488)
  • 宿主
    Rabbit
  • 偶联物
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • 经测试应用
    适用于: ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Common marmoset
  • 免疫原

    Synthetic peptide. within Human Glutathione Peroxidase 1 aa 150 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: P07203
    (Peptide available as ab156307)

  • 阳性对照
    • ICC/IF: Hek293 cells. Flow Cyt: Hek293 cells.
  • 常规说明

    Alternative versions available:
    Anti-Glutathione Peroxidase 1 antibody [EPR3312] (ab108427) Knockout validated

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

性能

应用

Our Abpromise guarantee covers the use of ab197527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.

This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min)

Flow Cyt 1/500.

靶标

  • 功能
    Protects the hemoglobin in erythrocytes from oxidative breakdown.
  • 序列相似性
    Belongs to the glutathione peroxidase family.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AL033363 antibody
    • Cellular glutathione peroxidase antibody
    • Glutathione peroxidase 1 antibody
    • Glutathione peroxidase antibody
    • GPx 1 antibody
    • GPx-1 antibody
    • GPX1 antibody
    • GPX1_HUMAN antibody
    • GPXD antibody
    • GSHPx-1 antibody
    • GSHPX1 antibody
    • MGC14399 antibody
    • MGC88245 antibody
    see all

图片

  • Overlay histogram showing HEK293 cells stained with ab197527 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197527, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab197527 staining Glutathione Peroxidase 1 in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197527 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

文献

This product has been referenced in:
  • Gao S  et al. MicroRNA-194 regulates cell viability and apoptosis by targeting CDH2 in prostatic cancer. Onco Targets Ther 11:4837-4844 (2018). Read more (PubMed: 30147335) »
See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab197527.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册