概述

  • 产品名称
    Anti-Glutamine Synthetase抗体
    参阅全部 Glutamine Synthetase 一抗
  • 描述
    兔多克隆抗体to Glutamine Synthetase
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Monkey
    预测可用于: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    RTCLLNETGDEPFQYKN

    , corresponding to C terminal amino acids 357-373 of Mouse Glutamine Synthetase

  • 阳性对照
    • Rat brain tissue, rat brain cytosolic fraction extract, cerebellum, kidney

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • 纯度
    Ion Exchange Chromatography
  • 纯化说明
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/10000 - 1/20000.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/5000.

靶标

  • 功能
    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • 疾病相关
    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • 序列相似性
    Belongs to the glutamine synthetase family.
  • 发展阶段
    Expressed during early fetal stages.
  • 细胞定位
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

图片

  • All lanes :

    Lane 2 : 10000
    Lane 3 : 20000

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase

    Predicted band size: 42 kDa

  • ab49873 at 1/5000 staining cultured mouse astroglial cells by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with TritonX100 before being blocked and stained with the antibody for 24 hours at 4°C. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit antibody was used as the secondary.

    See Abreview

  • Glutamine Synthetase antibody (ab49873) immunohistochemistry on rat brain and kidney tissue (formalin/PFA-fixed paraffin-embedded) sections. Rat cerebellum and rat kidney sections were incubated with ab49873 (1/21000) for 2h at RT. Antigen retrieval was performed by heat induction in citrate buffer pH6. The upper panel shows immunoreactivity in rat kidney, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows). In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes.
    .

    See Abreview

文献

This product has been referenced in:
  • Zhou M  et al. Non-cell-autonomous activation of IL-6/STAT3 signaling mediates FGF19-driven hepatocarcinogenesis. Nat Commun 8:15433 (2017). IHC-P ; Mouse . Read more (PubMed: 28508871) »
  • Sidoryk-Wegrzynowicz M  et al. Astrocytes in mouse models of tauopathies acquire early deficits and lose neurosupportive functions. Acta Neuropathol Commun 5:89 (2017). WB . Read more (PubMed: 29187256) »
See all 20 Publications for this product

客户评价及客户问答

1-8 of 8 Q&A

Answer

Thank you for your call today and for letting us know about this issue.
I've created a discount code that can be used to receive 20% off a vial of ab49873. Please include the code below with your order to redeem this offer:
Discount Code: ***
Expires: August 18, 2012
I hope that this will be helpful, but please let me know if you have any questions or if there is anything else that we can do for you. Have a nice day.

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Question
Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Wie am Telefon diskutiert, ist unseres Wissens nach wurde der ab49873 bisher noch nicht inauf humanem Gewebegetestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab49873 abdecken, und gegen eine erneute Bestellung eines weiterenprimären Antikörpersvon uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab49873 auf humanem Gewebetesten möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper auf humanem Gewebe.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderenprimären Antikörperbei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab49873 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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Answer

That's no problem. I am sorry for the confusion, I should have made it clear to you that they would be arriving in the same box.

If I can help in any other way please do let me know. Otherwise, I wish you all the best with your research.

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Question
Answer

That is no problem. If you have any problems in the future, please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

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Answer

Thank you for getting back to me.

I am sorry for the frustration that this situation has caused you. I will arrange for ab91073 to be sent out to you as discussed. I can also arrange for ab60709 to be sent as a replacement for ab49873 which you have been having problems with. If you could just confirm that the following address is correct:



As soon as I have this confirmation I will have the antibodies sent to you.

I look forward to receiving your reply.

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Answer

Thank you for getting back to me.

I am sorry that the reply to your fax was sent to your supervisor by one of my colleagues, Keith. This was due to the way our assigning system works. The protocol tips that I gave and those of Keith are not that dissimilar. We both recommended optimising the dilution of the antibody. Keith also recommended using Glycine to reduce the possible background created from uncapped aldehyde groups. I did not mention this as you were having problems seeing any staining, the background was therefore not my top priority to tackle. What may be significantly contributing to your lack of signal may be the way that the tissue is fixed. Over fixation can lead tothe epitopeto be probed to bemasked.Even with permiabilisation, thismay not be unmasked. Someform of antigen retrieval may therefore be necessary.In my previous email I tried to find out a little more about this in order to be able to provide advice on how this could be improved:

1. Exactly how was the tissue fixed? For how long was the tissue treated with PFA? What concentration of PFA was used? At what temperature?

2. Has the goat anti-rabbit secondary antibody used for both experiments been used successfully before?

3. With the tissue sections that you have, have they been successfully stained to detect a different target with a different antibody? Could you send me some images of this staining?

If you could also let me know under exactly what conditions the new experiment was carriedout, that would be helpful. Which ofmy suggestionsdid you try?

As I mentioned in my previous email, ab93434 has to our knowledge not been used in staining of frozen sections and it may be that the antibody is not suitable for this application. As this application is not listed on the datasheet the antibody is therefore not covered by the Abpromise for this application.

As you are having problems with both of these very different antibodies and I am as yet not sure what is contributing to this problem, it seems that it may either be the tissue being used or the protocol which may be contributing to the lack of signal. As I want you to get the best staining that you can, I would therefore like to ascertain if it is a problem of the antibodies first. Have you performed a western blotting experiment with these antibodies? If not, I would like to provide you with a positive control for this application to test the activity of the antibodies.

If you would like for me to send this positive control for western blotting please do let me know. Otherwise, all I can offerat this time is a refund, in addition to the ab91073 which I offered as a good-will gesture.

I hope this has been of help. I look forward to receivingyour reply.

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Answer

Thank you for providing me with this extra information. It has allowed me to more fully understand the problems you have been experiencing with ab49873 and ab93434 previously.

Having looked at the protocol I have a few further questions:

1. Exactly how was the tissue fixed? For how long was the tissue treated with PFA? What concentration of PFA was used?

2. What were the samples tested? Which species did the tissue come from?

3. Had the goat anti rabbit used for both of the experiments been used successfully before?

As ab93434 has as yet to our knowledge not been used in staining frozen sections it may be that the antibody is not suitable for this application. However, there are a few suggestions I can make which may help toimprove the results seen so far.

1. I would suggest decreasing the dilution of both the antibodies. Although ab49873 is recommended to be used at 1/5000, this is only a starting point and may need to be optimised depending on the tissue and protocol used. With ab49873, I would suggest initially trying 1/500 to see if there is any signal. With ab93434 we recommend using 5 µg/ml as an initial starting point, I would therefore use a dilution of 1/50 to start.

2. As you have not been observing any staining with overnight incubation at 4 degrees, I would try incubating at room temperature for 2 hours to see if this improves the results.

3. I would increase the level of Triton X used to 0.2 % for performing the permiabilisation.

I hope these protocol tips are of use, with the answers to the questions above I may be able to provide further suggestions.

I am able to send you ab91073 directly as I suggested to detect theL isoform of glutaminase as a good-will gesture for the bad experience you have had. If you would prefer to change this to ab60709 I can also organise this.

I look forward to receiving your reply.

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Question
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab49873 with the order number 972573. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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