重组Anti-Glutaminase抗体[EP7212] (ab156876)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP7212] to Glutaminase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Glutaminase抗体[EP7212]
参阅全部 Glutaminase 一抗 -
描述
兔单克隆抗体[EP7212] to Glutaminase -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human kidney tissue lysates, HeLa and 293T cell lysates, wild type HAP1 cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP7212 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab156876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 73 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 73 kDa. |
靶标
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功能
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. -
组织特异性
KGA is expressed predominantly in brain and kidney but not in liver, isoform 3 is expressed principally in cardiac muscle and pancreas but not in liver or brain, and isoform 2 is expressed solely in cardiac and skeletal muscle. -
序列相似性
Belongs to the glutaminase family.
Contains 1 ANK repeat. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 2744 Human
- Omim: 138280 Human
- SwissProt: O94925 Human
- Unigene: 116448 Human
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别名
- AAD20 antibody
- DKFZp686O15119 antibody
- FLJ10358 antibody
see all
图片
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All lanes : Anti-Glutaminase antibody [EP7212] (ab156876) at 1/1000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Glutaminase antibody [EP7212] (ab156876) at 1/1000 dilution
Lane 1 : Human kidney tissue lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 73 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Lane 1: Wild type HAP1 whole cell lysate (20 µg) Lane 2: GLS knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: HEK293 whole cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab156876 observed at 62 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab156876 was shown to recognize GLS in wild-type HAP1 cells as signal was lost at the expected MW in HAP1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and GLS knockout samples were subjected to SDS-PAGE. Ab156876 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.
Blue arrow corresponds to isoform 1 KGA.
Red arrow corresponds to isoform 3 GAC.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (77)
ab156876 被引用在 77 文献中.
- Chen W et al. Qici Sanling Decoction Suppresses Glutamine Consumption and Bladder Cancer Cell Growth through Inhibiting c-Myc Expression. J Oncol 2022:7985468 (2022). PubMed: 35058981
- Wang L et al. Spatially resolved isotope tracing reveals tissue metabolic activity. Nat Methods 19:223-230 (2022). PubMed: 35132243
- Huynh VN et al. Acute inhibition of OGA sex-dependently alters the networks associated with bioenergetics, autophagy, and neurodegeneration. Mol Brain 15:22 (2022). PubMed: 35248135
- Farah H et al. Differential Metabotypes in Synovial Fibroblasts and Synovial Fluid in Hip Osteoarthritis Patients Support Inflammatory Responses. Int J Mol Sci 23:N/A (2022). PubMed: 35328687
- Mohás A et al. In Situ Analysis of mTORC1/C2 and Metabolism-Related Proteins in Pediatric Osteosarcoma. Pathol Oncol Res 28:1610231 (2022). PubMed: 35392503