重组Anti-Glucose Transporter GLUT4 (phospho S488)抗体[EPR930(2)] (ab188317)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR930(2)] to Glucose Transporter GLUT4 (phospho S488)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Glucose Transporter GLUT4 (phospho S488)抗体[EPR930(2)]
参阅全部 Glucose Transporter GLUT4 一抗 -
描述
兔单克隆抗体[EPR930(2)] to Glucose Transporter GLUT4 (phospho S488) -
宿主
Rabbit -
经测试应用
适用于: WB, Dot blotmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal heart, mouse heart, rat heart
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR930(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab188317于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa).
We recommend not to boil the samples before loading to get desirable WB results. |
Dot blot |
Use at an assay dependent concentration.
|
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 55 kDa). We recommend not to boil the samples before loading to get desirable WB results. |
Dot blot
Use at an assay dependent concentration. |
靶标
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功能
Insulin-regulated facilitative glucose transporter. -
组织特异性
Skeletal and cardiac muscles; brown and white fat. -
疾病相关
Diabetes mellitus, non-insulin-dependent -
序列相似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
翻译后修饰
Sumoylated. -
细胞定位
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
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数据库链接
- Entrez Gene: 6517 Human
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- Omim: 138190 Human
- SwissProt: P14672 Human
- SwissProt: P14142 Mouse
- SwissProt: P19357 Rat
- Unigene: 380691 Human
see all -
别名
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
图片
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All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution
Lane 1 : Human heart lysate, boiled
Lane 2 : Human heart lysate, unboiled
Lane 3 : Mouse heart lysate, boiled
Lane 4 : Mouse heart lysate, unboiled
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Lanes 1 and 2: 7 seconds exposure
Lanes 3 and 4: 20 seconds exposure
Blocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/1000 dilution
Lane 1 : Human fetal heart whole cell lysate
Lane 2 : Human fetal heart whole cell lysate with Phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugated (DC03L) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Diluting buffer and concentration 5% NFDM/TBST
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Dot Blot analysis of Lane 1: Glucose Transporter GLUT4 (pS488) phospho peptide and Lane 2: Glucose Transporter GLUT4 non-phospho peptide labeling Glucose Transporter GLUT4 (phospho S488) with ab188317 at 1/1000 dilution.
5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.
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All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/5000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Glucose Transporter GLUT4 (phospho S488) antibody [EPR930(2)] (ab188317) at 1/20000 dilution
Lane 1 : Untreated human fetal heart
Lane 2 : Lambda phosphorylase treated human fetal heart
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (6)
ab188317 被引用在 6 文献中.
- Zhang R et al. An NF-κB/OVOL2 circuit regulates glucose import and cell survival in non-small cell lung cancer. Cell Commun Signal 20:40 (2022). PubMed: 35346238
- Rahimi G et al. A combination of herbal compound (SPTC) along with exercise or metformin more efficiently alleviated diabetic complications through down-regulation of stress oxidative pathway upon activating Nrf2-Keap1 axis in AGE rich diet-induced type 2 diabetic mice. Nutr Metab (Lond) 18:14 (2021). PubMed: 33468193
- Montt-Guevara MM et al. D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes. Front Endocrinol (Lausanne) 12:660815 (2021). PubMed: 33859622
- Zhuo J et al. Evaluation of type 2 diabetic mellitus animal models via interactions between insulin and mitogen-activated protein kinase signaling pathways induced by a high fat and sugar diet and streptozotocin. Mol Med Rep 17:5132-5142 (2018). PubMed: 29393432
- Wang N et al. Hydroxytyrosol ameliorates insulin resistance by modulating endoplasmic reticulum stress and prevents hepatic steatosis in diet-induced obesity mice. J Nutr Biochem 57:180-188 (2018). WB ; Mouse . PubMed: 29747118
- Liu Z et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis 7:e2487 (2016). WB ; Mouse . PubMed: 27882945