Anti-Glucose Transporter GLUT4抗体(ab654)
概述
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产品名称Anti-Glucose Transporter GLUT4抗体
参阅全部 Glucose Transporter GLUT4 一抗 -
描述兔多克隆抗体to Glucose Transporter GLUT4
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宿主Rabbit
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特异性GLUT4.
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经测试应用适用于: ICC/IF, WB, IP, Immunomicroscopy, IHC-Fr, IHC-Pmore details
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种属反应性与反应: Mouse, Rat, Human
预测可用于: Pig, Armenian hamster
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免疫原
Synthetic peptide corresponding to Rat Glucose Transporter GLUT4 aa 495-509 (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab115831) -
阳性对照
- IHC-P: FFPE human heart tissue sections.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: 0.01% Sodium azide
Constituent: Whole serum -
纯度Whole antiserum
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
应用
Our Abpromise guarantee covers the use of ab654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/1000. | |
| WB | 1/2000 - 1/2500. Detects a band of approximately 45 kDa (predicted molecular weight: 54.8 kDa).Can be blocked with Rat Glucose Transporter GLUT4 peptide (ab115831). The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed. It is recomended to use 3% milk block. |
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| IP | Use at an assay dependent concentration. PubMed: 18234908 | |
| Immunomicroscopy | Use at an assay dependent concentration. Can also can be used for Immunogold EM (purified IgG). See references for specific protocols. | |
| IHC-Fr | 1/500. | |
| IHC-P | Use at an assay dependent concentration. |
靶标
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功能Insulin-regulated facilitative glucose transporter.
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组织特异性Skeletal and cardiac muscles; brown and white fat.
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疾病相关Diabetes mellitus, non-insulin-dependent
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序列相似性Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
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翻译后修饰Sumoylated.
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细胞定位Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
- Information by UniProt
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数据库链接
- Entrez Gene: 6517 Human
- Entrez Gene: 20528 Mouse
- Entrez Gene: 25139 Rat
- Omim: 138190 Human
- SwissProt: P14672 Human
- SwissProt: P14142 Mouse
- SwissProt: P19357 Rat
- Unigene: 380691 Human
see all -
别名
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
图片
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Western blot - Anti-Glucose Transporter GLUT4 antibody (ab654)Image courtesy of an anonymous AbreviewAnti-Glucose Transporter GLUT4 antibody (ab654) at 1/1000 dilution (Diluted with TBST and Incubated for 16 hours at 4°C) + Mouse Heart at 30 µg with Milk, 1 hour at 25°C at 3 %
Secondary
Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) HRP at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 54.8 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)IHC image of Glucose Transporter GLUT4 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab654, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)This image is courtesy of Steven Johnstoneab654 staining GLUT4 in human mesenchymal stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% Horse serum) for 1 hour at 22°C. A FITC-conjugated rabbit polyclonal was used as the secondary antibody (1/100).
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Immunohistochemistry (Frozen sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)ab654 at a 1/300 dilution, staining human GLUT4 in fetal heart tissue by Immunohistochemistry, frozen tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)ICC/IF image of ab654 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab654, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)This image is courtesy of an abreview submitted by Brett McKinnonab654 staining GLUT4 in human endometriosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 3% BSA in TBS) for 16 hours at 4°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (Frozen sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)This image is courtesy of an anonymous Abreview.ab654 at 1/500 dilution staining GLUT4 in mouse muscle by immunohistochemistry (frozen sections). Sections were methanol fixed prior to blocking in 5% goat serum for 1 hour at 25°C and then incubated with ab654 for 1 hour at 25°C. A goat polyclonal to rabbit IgG, diluted 1/500, was used as the secondary antibody.
文献
This product has been referenced in:
- Zhang M et al. Semen Cassiae Extract Improves Glucose Metabolism by Promoting GlUT4 Translocation in the Skeletal Muscle of Diabetic Rats. Front Pharmacol 9:235 (2018). Read more (PubMed: 29670524) »
- Tetri LH et al. Sex-Specific Skeletal Muscle Fatigability and Decreased Mitochondrial Oxidative Capacity in Adult Rats Exposed to Postnatal Hyperoxia. Front Physiol 9:326 (2018). WB ; Rat . Read more (PubMed: 29651255) »
