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Signal Transduction Metabolism Energy Metabolism

Anti-Glucose Transporter GLUT4抗体(ab654)

  • Datasheet
  • SDS
Reviews (18)Q&A (35)References (145)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)

Key features and details

  • Rabbit polyclonal to Glucose Transporter GLUT4
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Glucose Transporter GLUT4抗体
    参阅全部 Glucose Transporter GLUT4 一抗
  • 描述

    兔多克隆抗体to Glucose Transporter GLUT4
  • 宿主

    Rabbit
  • 特异性

    GLUT4.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Rat Glucose Transporter GLUT4 aa 495-509 (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab115831)

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Rat Glucose Transporter GLUT4 peptide (ab115831)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC/IF
Human
IHC-P
Human
All applications
Pig
Armenian hamster
应用 Ab评论 说明
ICC/IF (1)
1/1000.
IHC-P (7)
Use at an assay dependent concentration.
说明
ICC/IF
1/1000.
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Insulin-regulated facilitative glucose transporter.
  • 组织特异性

    Skeletal and cardiac muscles; brown and white fat.
  • 疾病相关

    Diabetes mellitus, non-insulin-dependent
  • 序列相似性

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻译后修饰

    Sumoylated.
  • 细胞定位

    Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Target information above from: UniProt accession P14672 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6517 Human
    • Omim: 138190 Human
    • SwissProt: P14672 Human
    • Unigene: 380691 Human
    • 别名

      • insulin-responsive antibody
      • Glucose transporter GLUT 4 antibody
      • Glucose transporter type 4 antibody
      • Glucose transporter type 4 insulin responsive antibody
      • GLUT 4 antibody
      • GLUT-4 antibody
      • GLUT4 antibody
      • GTR4_HUMAN antibody
      • Insulin responsive glucose transporter type 4 antibody
      • kug antibody
      • SLC 2A4 antibody
      • SLC2A4 antibody
      • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
      • Solute carrier family 2 member 4 antibody
      • Solute carrier family 2, facilitated glucose transporter member 4 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)

      IHC image of Glucose Transporter GLUT4 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab654, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)This image is courtesy of Steven Johnstone

      ab654 staining GLUT4 in human mesenchymal stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% Horse serum) for 1 hour at 22°C. A FITC-conjugated rabbit polyclonal was used as the secondary antibody (1/100).

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)

      ICC/IF image of ab654 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab654, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)This image is courtesy of an abreview submitted by Brett McKinnon

      ab654 staining GLUT4 in human endometriosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 3% BSA in TBS) for 16 hours at 4°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (145)

    发表研究结果有使用 ab654?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab654 被引用在 145 文献中.

    • Esmailidehaj M  et al. 17ß-Estradiol improves insulin signalling and insulin resistance in the aged female hearts: Role of inflammatory and anti-inflammatory cytokines. Life Sci 253:117673 (2020). PubMed: 32311377
    • Tan YQ  et al. The livestock growth-promoter zeranol facilitates GLUT4 translocation in 3T3 L1 adipocytes. Chemosphere 253:126772 (2020). PubMed: 32464760
    • Zhu J  et al. Sevoflurane Induces Learning and Memory Impairment in Young Mice Through a Reduction in Neuronal Glucose Transporter 3. Cell Mol Neurobiol 40:879-895 (2020). PubMed: 31884568
    • Actis Dato V  et al. LRP1-Mediated AggLDL Endocytosis Promotes Cholesteryl Ester Accumulation and Impairs Insulin Response in HL-1 Cells. Cells 9:N/A (2020). PubMed: 31936892
    • Hofstee P  et al. Maternal Selenium Deficiency in Mice Alters Offspring Glucose Metabolism and Thyroid Status in a Sexually Dimorphic Manner. Nutrients 12:N/A (2020). PubMed: 31968625
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 53 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (stomach)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    stomach
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 06 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Ileum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Ileum
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 06 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Breast cancer
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 06 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Artery)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Artery
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 06 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    10mM Sodium Citrate pH6
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 06 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Glucose Transporter GLUT4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Placental Explant)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
    Permeabilization
    No
    Specification
    Placental Explant
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Jeremiah Gaudet

    Verified customer

    提交于 Aug 01 2017

    Western blot abreview for Anti-Glucose Transporter GLUT4 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse brain, heart, liver, muscle, and retina)
    Gel Running Conditions
    Reduced Denaturing (12% gel)
    Loading amount
    30 µg
    Specification
    Mouse brain, heart, liver, muscle, and retina
    Blocking step
    Licor Odyssey Blocking BUffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 23 2016

    Western blot abreview for Anti-Glucose Transporter GLUT4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart)
    Gel Running Conditions
    Reduced Denaturing (gel 4-15% SDS)
    Loading amount
    30 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 04 2015

    Question

    I would like to make a negative control but I have not been able to find an appropriate tissue to do this on. Therefor I was wondering if you have any good suggestions?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2014

    Answer

    I would recommend looking at the human protein atlas which shows in which tissues the protein is expressed:

    http://www.proteinatlas.org/ENSG00000181856/tissue

    From this, it would seem that the following tissues could be good negative controls:

    Liver, gallbladder, pancreas, oral mucosa, esophagus, duodenum, small intestine, appendix, rectum, epididymis, prostate, seminal vesicle, breast, vagina, cervix, uterus, fallopian tube, placenta, skin, bone marrow, lymph node, tonsil, spleen, thyroid gland, and nasopharynx.

    There therefore seems to be a large choice of negative control tissues but I would also recommend cross-checking these with the literature to see if they are true negative controls.

    We also sell the blocking peptide for ab654 which you could use as a second negative control: Rat Glucose Transporter GLUT4 peptide (ab115831)

    Read More

    Elisa Thomas

    Abcam Scientific Support

    回复于 Jul 18 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Sample
    Human Tissue sections (Endometriosis)
    Specification
    Endometriosis
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Dr. Brett Mckinnon

    Verified customer

    提交于 Jun 10 2013

    1-10 of 53 Abreviews or Q&A

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