Anti-Glucose Transporter GLUT4抗体(ab654)
Key features and details
- Rabbit polyclonal to Glucose Transporter GLUT4
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Glucose Transporter GLUT4抗体
参阅全部 Glucose Transporter GLUT4 一抗 -
描述
兔多克隆抗体to Glucose Transporter GLUT4 -
宿主
Rabbit -
特异性
GLUT4. -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P Human -
免疫原
Synthetic peptide corresponding to Rat Glucose Transporter GLUT4 aa 495-509 (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab115831)
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
ICC/IF |
Human
|
IHC-P |
Human
|
All applications |
Pig
Armenian hamster
|
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | (1) |
1/1000.
|
IHC-P | (7) |
Use at an assay dependent concentration.
|
说明 |
---|
ICC/IF
1/1000. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Insulin-regulated facilitative glucose transporter. -
组织特异性
Skeletal and cardiac muscles; brown and white fat. -
疾病相关
Diabetes mellitus, non-insulin-dependent -
序列相似性
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
翻译后修饰
Sumoylated. -
细胞定位
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
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数据库链接
- Entrez Gene: 6517 Human
- Omim: 138190 Human
- SwissProt: P14672 Human
- Unigene: 380691 Human
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别名
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)
IHC image of Glucose Transporter GLUT4 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab654, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT4 antibody (ab654)This image is courtesy of Steven Johnstone
ab654 staining GLUT4 in human mesenchymal stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% Horse serum) for 1 hour at 22°C. A FITC-conjugated rabbit polyclonal was used as the secondary antibody (1/100).
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ICC/IF image of ab654 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab654, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT4 antibody (ab654)This image is courtesy of an abreview submitted by Brett McKinnon
ab654 staining GLUT4 in human endometriosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 3% BSA in TBS) for 16 hours at 4°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (145)
ab654 被引用在 145 文献中.
- Esmailidehaj M et al. 17ß-Estradiol improves insulin signalling and insulin resistance in the aged female hearts: Role of inflammatory and anti-inflammatory cytokines. Life Sci 253:117673 (2020). PubMed: 32311377
- Tan YQ et al. The livestock growth-promoter zeranol facilitates GLUT4 translocation in 3T3 L1 adipocytes. Chemosphere 253:126772 (2020). PubMed: 32464760
- Zhu J et al. Sevoflurane Induces Learning and Memory Impairment in Young Mice Through a Reduction in Neuronal Glucose Transporter 3. Cell Mol Neurobiol 40:879-895 (2020). PubMed: 31884568
- Actis Dato V et al. LRP1-Mediated AggLDL Endocytosis Promotes Cholesteryl Ester Accumulation and Impairs Insulin Response in HL-1 Cells. Cells 9:N/A (2020). PubMed: 31936892
- Hofstee P et al. Maternal Selenium Deficiency in Mice Alters Offspring Glucose Metabolism and Thyroid Status in a Sexually Dimorphic Manner. Nutrients 12:N/A (2020). PubMed: 31968625