Anti-Glucose Transporter GLUT2抗体[5D1] (ab85715)


  • 产品名称
    Anti-Glucose Transporter GLUT2抗体[5D1]
    参阅全部 Glucose Transporter GLUT2 一抗
  • 描述
    小鼠单克隆抗体[5D1] to Glucose Transporter GLUT2
  • 宿主
  • 特异性
    ab85715 recognizes Glucose Transporter GLUT2 expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the Human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3.
  • 经测试应用
    适用于: IHC-P, ICC, Flow Cyt, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human Glucose Transporter GLUT2 transfected NS0 cells.

  • 阳性对照
    • Liver tissue.


Our Abpromise guarantee covers the use of ab85715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
ICC Use a concentration of 10 µg/ml.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IHC-FoFr Use at an assay dependent concentration. PubMed: 21857924
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).


  • 功能
    Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
  • 组织特异性
    Liver, insulin-producing beta cell, small intestine and kidney.
  • 疾病相关
    Fanconi-Bickel syndrome
  • 序列相似性
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻译后修饰
    N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • liver antibody
    • Glucose Transporter 2 antibody
    • Glucose Transporter GLUT2 antibody
    • Glucose transporter type 2 antibody
    • Glucose transporter type 2 liver antibody
    • Glucose transporter, liver/islet antibody
    • GLUT-2 antibody
    • GLUT2 antibody
    • GTR2_HUMAN antibody
    • GTT2 antibody
    • SLC2A2 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 2 antibody
    • Solute carrier family 2 facilitated glucose transporter member 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 2 antibody
    see all


  • ab85715, at 10 µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded human Liver tissue by Immunohistochemistry.
  • Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

    Exposure time: 8 minutes
  • Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemical analysis of frozen pancreatic tissue in control (left) or TAT-MafA mutant (right) neonate mice.

    Glucose Transporter GLUT2 was stained using ab85715 (green), at 10 mg/ml. An AlexaFluor®647-conjugated donkey anti-mouse IgG was used as the secondary antibody.


This product has been referenced in:
  • Fujino M  et al. Expression of glucose transporter-1 is correlated with hypoxia-inducible factor 1a and malignant potential in pancreatic neuroendocrine tumors. Oncol Lett 12:3337-3343 (2016). IHC-P ; Human . Read more (PubMed: 27900001) »
  • Mano Y  et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . Read more (PubMed: 25125631) »

See all 4 Publications for this product


Thank you for your reply and also for checking the lysate. This is really helpful.

I am sorry to hear that the problem has not been resolved.
Since you see no protein bands before transfer, it does not surprise me that you see none after...

Read More

Thank you for contacting us.
I am sorry to hear about the problem the pancreas lysate is causing you.

As we discussed over the phone, it would be worthwhile trying the following in order to determine the cause of the problem:


Read More

Vielen Dank für Ihre Anfrage.

Leider haben wir keinen Antikörper, der allen genannten Anforderungen entspricht.

Wir führen einen Maus-monoklonalen GLUT2-Antikörper, der bislang nur für humane Proben getestet wurde (ab85715). Das I...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (Pancreas)
Yes - PBST (0.02% Tween)
Blocking step
(agent) for 10 minute(s) · Concentration: 100% · Temperature: 25°C

Mr. Tirosh Shapira

Verified customer

提交于 Mar 16 2010