Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (OCT preserved Glioblastoma Spheroid)
Permeabilization
Yes - Triton 0.5%
Specification
OCT preserved Glioblastoma Spheroid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% goat serum
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L) Cross-Adsorb
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Maria C Johansson
Verified customer
提交于 Sep 07 2021