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重组
RabMAb

重组Anti-Glucose Transporter GLUT1抗体[EPR3915] (ab115730)

概述

  • 产品名称

    Anti-Glucose Transporter GLUT1抗体[EPR3915]
    参阅全部 Glucose Transporter GLUT1 一抗
  • 描述

    兔单克隆抗体[EPR3915] to Glucose Transporter GLUT1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WB, IHC-P, Flow Cytmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    corresponding to Human Glucose Transporter GLUT1 aa 450 to the C-terminus.
    (Peptide available as ab202335)

  • 阳性对照

    • WB: NIH/3T3, HepG2, HT-29, SW480, 3T3-L1 and PC-12 whole cell lysates; IHC-P: Rat kidney tissue; mouse liver tissue; human lung carcinoma, cervical carcinoma, colon carcinoma, liver, colon, kidney carcinoma, skeletal muscle, urinary bladder, heart and breast tissue. ICC/IF: HepG2 cells Flow cyt: HeLa and Jurkat cells.
  • 常规说明

      

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab115730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/500.
WB 1/100000. Detects a band of approximately 40-60 kDa (predicted molecular weight: 54 kDa).Can be blocked with Glucose Transporter GLUT1 peptide (ab202335).

We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading.

IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt 1/40.

For unpurified, use 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • 组织特异性

    Expressed at variable levels in many human tissues.
  • 疾病相关

    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • 序列相似性

    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • 翻译后修饰

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位

    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接

  • 别名

    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

图片

  • All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1 µg/ml

    Lane 1 : Wild-type A549 whole cell lysate
    Lane 2 : SLC2A1 knockout A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 54 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab196357 observed at 54 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab196357 was shown to recognize in wild-type A549 cells as signal was lost at the expected MW in SLC2A1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SLC2A1 knockout samples were subjected to SDS-PAGE. Ab196357 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • GLUT1 and GLUT3 are downregulated in KSHV-infected cells in human KS tumors

    Representative illustration of dual immunofluorescence detection of LANA and GLUT1 or in a normal human skin section and a Karposi Sarcoma (KS) tumor section. Tissues were fixed with paraformaldehyhde and paraffin-embedded.

     

  • Immunohistochemical expression of Glut1 in normal tongue epithelium and tongue cancer. Expression was greatest in lymphocytes (arrows in left upper and lower panels). In the normal oral epithelium, Glut1 was weakly expressed in the basal and spinous cells (left upper panel). In OSCC, Glut1 was upregulated, showing a level of expression comparable with lymphocytes (left and right lower panels). Scale bar, 100 μm.

    Note: Glut1 = SLC2A (alternative names for the same target).

  • All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000000 dilution

    Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates unboiled with 5% NFDM/TBST
    Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates boiled with 5% NFDM/TBST
    Lane 3 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates unboiled with 5% NFDM/TBST
    Lane 4 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates boiled with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 54 kDa
    Observed band size: 40-60 kDa
    why is the actual band size different from the predicted?



    Exposure time

    Lane 1 to 2: 10 seconds
    Lane 3 to 4: 30 seconds

    We recommend not to boil the samples after lysis to get desired WB bands.

  • Immunohistochemical staining of paraffin embedded rat kidney with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunofluorescence staining of HepG2 cells with purified ab115730 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab115730 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Overlay histogram showing Jurkat cells fixed in 4% PFA and stained with purified ab115730 at a dilution of 1/40 (red line). The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

  • Immunohistochemical staining of paraffin embedded mouse liver with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human lung carcinoma with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab115730 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000000 dilution (purified)

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : HT-29 whole cell lysate
    Lane 4 : SW480 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 40-60 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730) at 1/1000 dilution (Unpurified)

    Lane 1 : Jurkat lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Human fetal brain lysate
    Lane 4 : 3T3L1 lysate
    Lane 5 : Human fetal liver lysate
    Lane 6 : HepG2 lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 54 kDa

  • Overlay histogram showing HeLa cells stained with unpurified ab115730 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115730, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human cervical carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab115730 at 1/250 dilution staining Glucose Transporter GLUT1 in Paraffin-embedded human colonic adenocarcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab115730 showing positive staining in normal liver tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab115730 showing positive staining in normal breast tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab115730 showing positive staining in normal colon tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab115730 showing positive staining in kidney carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab115730 showing negative staining in skeletal muscle tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab115730 showing positive staining in urinary bladder transitional carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Unpurified ab115730 showing negative staining in normal heart tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

文献

This product has been referenced in:

  • Martinez CA  et al. Obstructive Sleep Apnea Activates HIF-1 in a Hypoxia Dose-Dependent Manner in HCT116 Colorectal Carcinoma Cells. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30669593) »
  • Oliveira H  et al. GLUT1 and GLUT3 involvement in anthocyanin gastric transport- Nanobased targeted approach. Sci Rep 9:789 (2019). Read more (PubMed: 30692585) »
See all 58 Publications for this product

客户评价及客户问答

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1-10 of 13 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Term human placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate (pH 6.0)
Permeabilization
No
Specification
Term human placenta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Aug 29 2019

Application
Western blot
Sample
Dog Cell lysate - whole cell (melanoma cell line)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
melanoma cell line
Blocking step
BlockAce as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C

Dr. 令 中野

Verified customer

提交于 Jan 23 2018

Application
Western blot
Sample
Cat Cell lysate - whole cell (fibroblasts)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
fibroblasts
Blocking step
(agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jun 20 2019

Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Retina
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 04 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain, heart, liver, muscle)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
Mouse brain, heart, liver, muscle
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Oct 03 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Jul 25 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
HUVEC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Jul 25 2016

Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (ovary)
Gel Running Conditions
Reduced Denaturing (12.5)
Loading amount
50 µg
Specification
ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Daniel Wang

Verified customer

提交于 Feb 24 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Sample
Rat Tissue sections (Liver cancer)
Specification
Liver cancer
Permeabilization
Yes - Wash Buffer from Dako with tween
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

提交于 Mar 19 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with tween

Mr. Rudolf Jung

Verified customer

提交于 Apr 16 2013

1-10 of 13 Abreviews

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