Glucose Assay试剂盒- reducing agent compatible (ab102517)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.02 mM
概述
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产品名称
Glucose Assay试剂盒- reducing agent compatible
参阅全部 Glucose 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 0.02 mM -
范围
0.02 mM - 10 mM -
检测时间
0h 30m -
产品概述
Glucose Assay Kit ab102517 provides direct measurement of glucose in biological samples. It is particularly suitable for serum and urine samples since it is unaffected by reducing substances, which can interfere with detection in oxidase-based kits.
In the glucose assay protocol, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration.
The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes.
The kit can detect glucose concentrations in the range of 20µM-10mM.
Glucose assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader -
说明
This product is manufactured by BioVision, an Abcam company and was previously called K686 Glucose Colorimetric Assay Kit II. K686-100 is the same size as the 100 test size of ab102517.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer XXXIV 1 x 25ml Developer Solution III 1 unit Development Enzyme Mix V 1 unit Glucose Standard 1 x 100µl -
研究领域
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相关性
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
图片
数据表及文件
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SDS download
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Datasheet download
文献 (38)
ab102517 被引用在 38 文献中.
- Kulkarni A et al. A Novel 2-Hit Zebrafish Model to Study Early Pathogenesis of Non-Alcoholic Fatty Liver Disease. Biomedicines 10:N/A (2022). PubMed: 35203687
- Wurster JI et al. Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis. Cell Rep 37:110113 (2021). PubMed: 34910917
- Wang F et al. Platelet isoform of phosphofructokinase promotes aerobic glycolysis and the progression of non-small cell lung cancer. Mol Med Rep 23:N/A (2021). PubMed: 33236133
- Chen J et al. Chrysin serves as a novel inhibitor of DGKa/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). Acta Pharm Sin B 11:143-155 (2021). PubMed: 33532186
- Wang Y et al. Biological Significance of 18F-FDG PET/CT Maximum Standard Uptake Value for Predicting EGFR Mutation Status in Non-Small Cell Lung Cancer Patients. Int J Gen Med 14:347-356 (2021). PubMed: 33568935