Anti-AMF抗体(ab227472)
Key features and details
- Rabbit polyclonal to AMF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-AMF抗体
参阅全部 AMF 一抗 -
描述
兔多克隆抗体to AMF -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Cow, Pig, Chimpanzee -
免疫原
Recombinant fragment within Human AMF (internal sequence). The exact sequence is proprietary.
Database link: P06744 -
阳性对照
- WB: Raji and NCI-H1299 whole cell lysate. IHC-P: NCI-N87 xenograft tissue. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab227472于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 63 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/5000 - 1/20000. Predicted molecular weight: 63 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. -
通路
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. -
疾病相关
Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. -
序列相似性
Belongs to the GPI family. -
翻译后修饰
Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
ISGylated. -
细胞定位
Cytoplasm. Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 2821 Human
- Omim: 172400 Human
- SwissProt: P06744 Human
- Unigene: 466471 Human
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别名
- AMF antibody
- Aurocrine motility factor antibody
- Autocrine motility factor antibody
see all
图片
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All lanes : Anti-AMF antibody (ab227472) at 1/10000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 63 kDa7.5% SDS-PAGE
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Paraffin-embedded NCI-N87 xenograft tissue stained for AFM with ab227472 at 1/500 dilution in immunohistochemical analysis.
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HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AFM (green) using ab227472 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Blue: Hoechst 33342 staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab227472 尚未被引用在任何文献中。