Glucose 6 Phosphate Dehydrogenase Activity Assay试剂盒(Fluorometric) (ab176722)

概述

  • 产品名称
    Glucose 6 Phosphate Dehydrogenase Activity Assay试剂盒(Fluorometric)
    参阅全部 Glucose 6 Phosphate Dehydrogenase 试剂盒
  • 检测方法
    Fluorescent
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Whole Blood, Cell Lysate
  • 检测类型
    Quantitative
  • 灵敏度
    >= 0.3 mIU/ml
  • 检测时间
    1h 00m
  • 种属反应性
    与反应: Other species, Mammals
  • 产品概述

    Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722) provides a simple method for detecting glucose 6 phosphate dehydrogenase (G6PD) activity in a variety of biological samples such as serum, plasma and urine, as well as cell extracts. In the assay, G6PD present in the sample converts NADP+ to NADPH, which can be specifically monitored by a fluorogenic NADPH sensor to yield a highly red fluorescence product. The signal can be easily detected at Ex/Em = 540/590 nm in a fluorescent microplate reader. The fluorescence intensity is proportional to the G6PD activity present in the sample.


    The assay can detect glucose 6 phosphate dehydrogenase activity from as low as 0.3 mU/mL in a 100 µL reaction volume.

  • 说明

    Glucose 6 Phosphate Dehydrogenase (G6PD, G6PDH, EC 1.1.1.49) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway (PPP). It is a critical metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The production of NADPH is of great importance for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. The NADPH also maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Deficiencies in G6PD predispose individuals to non-immune hemolytic anemia.

  • 平台
    Microplate

性能

图片

  • G6PD was measured with Abcam's Glucose 6 Phosphate Dehydrogenase Assay Kit (Fluorometric) (ab176722) in a 96-well black plate using a microplate reader. As low as 0.3 mU/mL Glucose 6 Phosphate Dehydrogenase in 100 µL volume can be detected with 1 hour incubation.

实验方案

文献

ab176722 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab176722.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册