Application
Western blot
Sample
Pig Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
5 µg
Specification
Liver
Blocking step
skimmed milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% skimmed milk in 0,5% Triton-PBS
Dilution
1/2000
Secondary antibody
Dilution
1/40000
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ultra-sensitive ECL
Exposure
40 second(s)
Bands
Specific: ~95 kDa, ~70 kDa kDa
Additional data
Additional Notes
WB legend
1. liver homogenate, pig, 5 µg
2. liver homogenate, pig, 10 µg
3. liver homogenate, pig, 15 µg
4. liver homogenate, pig, 20µg
5. protein molecular weight marker
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Liliana Kiczak
Verified customer
提交于 Feb 23 2021