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Signal Transduction Signaling Pathway Nuclear Signaling Nuclear Hormone Receptors Corticoid

Anti-Glucocorticoid Receptor抗体(ab3579)

  • Datasheet
Reviews (8)Q&A (8)References (23)

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Western blot - Anti-Glucocorticoid Receptor antibody (ab3579)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579)
  • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)

Key features and details

  • Rabbit polyclonal to Glucocorticoid Receptor
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Glucocorticoid Receptor抗体
    参阅全部 Glucocorticoid Receptor 一抗
  • 描述

    兔多克隆抗体to Glucocorticoid Receptor
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 245-259.
    Sequence:

    CKPLILPDTKPKIKD


    (Peptide available as ab5833)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituent: 100% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Corticoid
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Corticoid
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Other

相关产品

  • Assay kits

    • Glucocorticoid Receptor Transcription Factor Assay Kit (Colorimetric) (ab207207)
  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • Glucocorticoid Receptor peptide (ab5833)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Glucocorticoid Receptor protein (ab82089)

应用

Our Abpromise guarantee covers the use of ab3579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Glucocorticoid Receptor peptide (ab5833). Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate.
ICC/IF 1/20.

靶标

  • 功能

    Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation.
  • 组织特异性

    Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.
  • 疾病相关

    Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.
  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰

    Increased proteasome-mediated degradation in response to glucocorticoids.
    Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
    Sumoylated; this reduces transcription transactivation.
    Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling.
  • 细胞定位

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus.
  • Target information above from: UniProt accession P04150 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 2908 Human
    • Omim: 138040 Human
    • SwissProt: P04150 Human
    • Unigene: 122926 Human
    • 别名

      • GCCR antibody
      • GCR antibody
      • GCR_HUMAN antibody
      • GCRST antibody
      • glucocorticoid nuclear receptor variant 1 antibody
      • Glucocorticoid receptor antibody
      • GR antibody
      • GRL antibody
      • Grl1 antibody
      • nr3c1 antibody
      • Nuclear receptor subfamily 3 group C member 1 antibody
      • nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
      see all

    图片

    • Western blot - Anti-Glucocorticoid Receptor antibody (ab3579)
      Western blot - Anti-Glucocorticoid Receptor antibody (ab3579)
      All lanes : Anti-Glucocorticoid Receptor antibody (ab3579) at 1 µg/ml

      Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg/ml with 5% Milk in TBST
      Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST
      Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 20 µg with 5% Milk in TBST
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST
      Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg with 5% Milk in TBST

      Secondary
      All lanes : HRP conjugate at 1/1000 dilution

      Predicted band size: 97 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579)

      Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control. 

    • Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)
      Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)

      Immunofluorescent analysis of ab3579 shows staining in HeLa (Human epithelial adenocarcinoma cell line) cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab3579at a dilution of 1/100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (23)

    发表研究结果有使用 ab3579?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3579 被引用在 23 文献中.

    • Wang C  et al. Extensive epigenomic integration of the glucocorticoid response in primary human monocytes and in vitro derived macrophages. Sci Rep 9:2772 (2019). PubMed: 30809020
    • Lee HA  et al. Histone deacetylase inhibition ameliorates hypertension and hyperglycemia in a model of Cushing's syndrome. Am J Physiol Endocrinol Metab 314:E39-E52 (2018). PubMed: 28928236
    • Tobin SW  et al. Regulation of Hspb7 by MEF2 and AP-1: implications for Hspb7 in muscle atrophy. J Cell Sci 129:4076-4090 (2016). PubMed: 27632998
    • Li Y  et al. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study. PLoS Med 13:e1002200 (2016). WB ; Human . PubMed: 27997540
    • Park OH  et al. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev 30:2093-2105 (2016). PubMed: 27798850
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 16 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glucocorticoid Receptor antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Heart
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 20 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Glucocorticoid Receptor antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.05% Tween 20
    Specification
    Heart
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 20 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-Glucocorticoid Receptor antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Permeabilization
    Yes - Triton
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Jacky Liang

    Verified customer

    提交于 Feb 12 2020

    Question

    Thanks a lot for the quick response.
    I would keep you informed about the observations.
    Adding up to the previous results I would also like to inform you that I gave another try in IHC including this time a citrate buffer antigen retreival step and still the result was not the usual expected one. I attach here the image obtained. In green are the pyramidal neurons of CA1- CA2 region of hippocampus and red depicts the GR antibody wich should show strong nuclear localization in CA1 layer.
    Hope that would be useful to you :-)

    Read More

    Abcam community

    Verified customer

    Asked on May 24 2012

    Answer

    Thank you very much for this extra information, this is very useful indeed for our investigations.

    Please do not hesitate to inform me if the replacement antibody gave you satisfactory results or not.

    Read More

    Abcam Scientific Support

    回复于 May 24 2012

    Question

    I would appreciate a replacement with ab 3579

    Read More

    Abcam community

    Verified customer

    Asked on May 23 2012

    Answer

    Thank you foryour cooperation.

    I am sorry ab3578 did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with one unit of ab3579. The order number ****.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 May 23 2012

    Question

    Dear Tech Support Team,



    Kindly advise the recommended protocol (dilution )for ChIP with ab3579. The customer intends to work with MOLT4 cells (human T cells).



    Thanks in advance for your assistance.



    Kind Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 03 2012

    Answer

    Thank you for your enquiry and your interest in our products.
    This antibody has not been characterized for ChIP in our lab. The ChIP application has been added to the datasheet based on this specific article:
    http://www.ncbi.nlm.nih.gov/pubmed?term=14522952
    There is another recent publication about this product; your customer may wish to take a look at it:
    O'Brien KB et al. CARM1 is required for proper control of proliferation and differentiation of pulmonary epithelial cells. Development 137:2147-56 (2010). ChIP; Mouse. PubMed: 20530543
    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Apr 03 2012

    ChIP abreview for Anti-Glucocorticoid Receptor antibody - ChIP Grade

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (LNCaP-1F5 Cell line)
    Specification
    LNCaP-1F5 Cell line
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: Formaldehyde
    Detection step
    Real-time PCR
    Positive control
    PSA enhancer ~4 kb upstream of TSS
    Negative control
    Negative control region on Chromosome X not known to be bound by any nuclear receptors.
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 02 2010

    Western blot abreview for Anti-Glucocorticoid Receptor antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LNCaP-1F5 Cell line and A549)
    Loading amount
    40 µg
    Specification
    LNCaP-1F5 Cell line and A549
    Treatment
    Ethanol, 100 nM Dexamethasone, in LNCaP-1F5 and A549 - 24 hours
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: +4°C
    Read More

    Dr. Biswajyoti Sahu

    Verified customer

    提交于 Nov 05 2009

    Western blot abreview for Anti-Glucocorticoid Receptor antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Neurons)
    Loading amount
    20 µg
    Specification
    Neurons
    Gel Running Conditions
    Reduced Denaturing (8% PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 26 2008

    Immunoprecipitation abreview for Anti-Glucocorticoid Receptor antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Tissue lysate - whole (skin epidermis)
    Total protein in input
    500 µg
    Specification
    skin epidermis
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 06 2008

    1-10 of 16 Abreviews or Q&A

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