Anti-Glucocorticoid Receptor抗体(ab3579)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Glucocorticoid Receptor抗体
参阅全部 Glucocorticoid Receptor 一抗 -
描述
兔多克隆抗体to Glucocorticoid Receptor -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 245-259.
Sequence:CKPLILPDTKPKIKD
(Peptide available asab5833)
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: 100% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 5 µg/ml. Predicted molecular weight: 97 kDa.Can be blocked with Glucocorticoid Receptor peptide (ab5833). Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate. | |
| ICC/IF | 1/20. |
靶标
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功能
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
组织特异性
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
疾病相关
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
序列相似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻译后修饰
Increased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2908 Human
- Omim: 138040 Human
- SwissProt: P04150 Human
- Unigene: 122926 Human
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别名
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
图片
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Western blot - Anti-Glucocorticoid Receptor antibody (ab3579)All lanes : Anti-Glucocorticoid Receptor antibody (ab3579) at 1 µg/ml
Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg/ml with 5% Milk in TBST
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg with 5% Milk in TBST
Secondary
All lanes : HRP conjugate at 1/1000 dilution
Predicted band size: 97 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579)Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
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Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)Immunofluorescent analysis of ab3579 shows staining in HeLa (Human epithelial adenocarcinoma cell line) cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab3579at a dilution of 1/100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
实验方案
数据表及文件
文献 (23)
ab3579 被引用在 23 文献中.
- Wang C et al. Extensive epigenomic integration of the glucocorticoid response in primary human monocytes and in vitro derived macrophages. Sci Rep 9:2772 (2019). PubMed: 30809020
- Lee HA et al. Histone deacetylase inhibition ameliorates hypertension and hyperglycemia in a model of Cushing's syndrome. Am J Physiol Endocrinol Metab 314:E39-E52 (2018). PubMed: 28928236
- Tobin SW et al. Regulation of Hspb7 by MEF2 and AP-1: implications for Hspb7 in muscle atrophy. J Cell Sci 129:4076-4090 (2016). PubMed: 27632998
- Li Y et al. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study. PLoS Med 13:e1002200 (2016). WB ; Human . PubMed: 27997540
- Park OH et al. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev 30:2093-2105 (2016). PubMed: 27798850
